Partially deglycosylated equine LH preferentially activates beta-arrestin-dependent signaling at the follicle-stimulating hormone receptor.

Molecular Endocrinology
Vanessa WehbiEric Reiter

Abstract

Deglycosylated FSH is known to trigger poor Galphas coupling while efficiently binding its receptor. In the present study, we tested the possibility that a deglycosylated equine LH (eLHdg) might be able to selectively activate beta-arrestin-dependent signaling. We compared native eLH to an eLH derivative [i.e. truncated eLHbeta (Delta121-149) combined with asparagine56-deglycosylated eLHalpha (eLHdg)] previously reported as an antagonist of cAMP accumulation at the FSH receptor (FSH-R). We confirmed that, when used in conjunction with FSH, eLHdg acted as an antagonist for cAMP accumulation in HEK-293 cells stably expressing the FSH-R. Furthermore, when used alone at concentrations up to 1 nM, eLHdg had no detectable agonistic activity on cAMP accumulation, protein kinase A activity or cAMP-responsive element-dependent transcriptional activity. At higher concentrations, however, a weak agonistic action was observed with eLHdg, whereas eLH led to robust responses whatever the concentration. Both eLH and eLHdg triggered receptor internalization and led to beta-arrestin recruitment. Both eLH and eLHdg triggered ERK and ribosomal protein (rp) S6 phosphorylation at 1 nM. The depletion of endogenous beta-arrestins had only a partial e...Continue Reading

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Citations

Apr 29, 2011·Reviews in Endocrine & Metabolic Disorders·Alfredo Ulloa-AguirreEric Reiter
Jul 9, 2011·Reviews in Endocrine & Metabolic Disorders·George R Bousfield, James A Dias
Apr 9, 2014·Journal of Molecular Endocrinology·Kelly LeónPascale Crepieux
Sep 18, 2015·Frontiers in Endocrinology·Mohammed Akli AyoubEric Reiter
Oct 19, 2016·Molecular Pharmacology·Alfredo Ulloa-Aguirre, Teresa Zariñán
Apr 12, 2015·General and Comparative Endocrinology·Hila AsrafDavid Ben-Menahem
Feb 9, 2017·Molecular and Cellular Endocrinology·Eric ReiterPascale Crépieux
Jul 10, 2018·Endocrinology·Alfredo Ulloa-AguirrePascale Crépieux
Jul 13, 2018·Minerva ginecologica·Francesco De Pascali, Eric Reiter
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Nov 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aurélie TréfierPascale Crépieux
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Dec 1, 2020·The FEBS Journal·Antara A BanerjeeSmita D Mahale
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