Partially folded aggregation intermediates of human gammaD-, gammaC-, and gammaS-crystallin are recognized and bound by human alphaB-crystallin chaperone.

Journal of Molecular Biology
Ligia Acosta-Sampson, Jonathan King

Abstract

Human gamma-crystallins are long-lived, unusually stable proteins of the eye lens exhibiting duplicated, double Greek key domains. The lens also contains high concentrations of the small heat shock chaperone alpha-crystallin, which suppresses aggregation of model substrates in vitro. Mature-onset cataract is believed to represent an aggregated state of partially unfolded and covalently damaged crystallins. Nonetheless, the lack of cell or tissue culture for anucleate lens fibers and the insoluble state of cataract proteins have made it difficult to identify the conformation of the human gamma-crystallin substrate species recognized by human alpha-crystallin. The three major human lens monomeric gamma-crystallins, gammaD, gammaC, and gammaS, all refold in vitro in the absence of chaperones, on dilution from denaturant into buffer. However, off-pathway aggregation of the partially folded intermediates competes with productive refolding. Incubation with human alphaB-crystallin chaperone during refolding suppressed the aggregation pathways of the three human gamma-crystallin proteins. The chaperone did not dissociate or refold the aggregated chains under these conditions. The alphaB-crystallin oligomers formed long-lived stable com...Continue Reading

References

Mar 1, 1976·Experimental Eye Research·D Roy, A Spector
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Horwitz
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·A Tardieu
Apr 1, 1969·Experimental Eye Research·R ClarkS Lerman
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T D Ingolia, E A Craig
May 1, 1994·Trends in Biotechnology·R Wetzel
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Apr 28, 2001·Biophysical Journal·J T Vivian, P R Callis
Feb 20, 2003·Protein Science : a Publication of the Protein Society·Melissa S Kosinski-Collins, Jonathan King
Mar 24, 2004·Seminars in Cell & Developmental Biology·José M BarralF Ulrich Hartl
May 12, 2004·Biochimica Et Biophysica Acta·Vladimir N Uversky, Anthony L Fink
Jul 27, 2004·Protein Science : a Publication of the Protein Society·Melissa S Kosinski-CollinsJonathan King
Aug 11, 2004·Progress in Biophysics and Molecular Biology·Hans BloemendalAnnette Tardieu
Nov 24, 2004·The International Journal of Developmental Biology·Jochen Graw
Aug 2, 2005·Biochemical and Biophysical Research Communications·Wangwang JiaoZengyi Chang
Mar 8, 2006·Biochemistry·Kirsten J LampiEric A Steel
Nov 7, 2006·IUBMB Life·G Bhanuprakash ReddyM Satish Kumar
Dec 26, 2006·Cellular and Molecular Life Sciences : CMLS·H Nakamoto, L Vígh
Sep 11, 2007·Journal of Proteome Research·Peter G Hains, Roger J W Truscott
Jan 30, 2008·The Journal of Biological Chemistry·Puttur SanthoshkumarK Krishna Sharma
Sep 4, 2008·IUBMB Life·Heath Ecroyd, John A Carver
Jun 23, 2009·Current Protein & Peptide Science·Elizabeth M BadenMarina Ramirez-Alvarado

❮ Previous
Next ❯

Citations

Sep 27, 2012·Cell Stress & Chaperones·Kelly M KneeJonathan A King
Mar 13, 2014·Progress in Biophysics and Molecular Biology·Kirsten J LampiLarry L David
Oct 5, 2013·The Journal of Chemical Physics·Benjamin G MohrMurugappan Muthukumar
Apr 25, 2013·The Journal of Biological Chemistry·Oksana A SergeevaJonathan A King
Aug 8, 2013·Journal of Immunotoxicology·Kirsty D RatanjiIan Kimber
Oct 27, 2012·Archives of Biochemistry and Biophysics·Maksym Tsytlonok, Laura S Itzhaki
Apr 24, 2012·Trends in Molecular Medicine·Kate L Moreau, Jonathan A King
Mar 13, 2012·The International Journal of Biochemistry & Cell Biology·Alice R ClarkChristine Slingsby
May 24, 2011·Experimental Eye Research·Daniel R GouletJonathan A King
Dec 10, 2015·Bioanalysis·Yan J ZhangDharmesh D Desai
Oct 29, 2010·Protein Science : a Publication of the Protein Society·Kelly M KneeJonathan A King
May 21, 2013·Photochemistry and Photobiology·Nathaniel Schafheimer, Jonathan King
Mar 13, 2014·Protein Science : a Publication of the Protein Society·Oksana A SergeevaKelly M Knee
Apr 5, 2016·Experimental Eye Research·Stephen Barnes, Roy A Quinlan
Jan 1, 2015·Nature Reviews. Disease Primers·Dennis LamDavid F Chang
Dec 3, 2014·International Journal of Biological Macromolecules·Md Anzarul HaqueFaizan Ahmad
May 18, 2016·International Journal of Biological Macromolecules·Mohammad Aasif DarFaizan Ahmad
Aug 21, 2013·Biochemistry·Sean D MoranMartin T Zanni
May 26, 2017·PloS One·Sibel CetinelCarlo Montemagno
Apr 21, 2018·The FEBS Journal·Takumi TakataNoriko Fujii
Jan 7, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Priyanka ChauhanKalyan Sundar Ghosh
Aug 16, 2014·The Journal of Biological Chemistry·Oksana A SergeevaJonathan A King
Sep 1, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·J A Domínguez-CalvaL Quintanar
May 31, 2019·Biomedical Optics Express·Alexander CuadradoJose Antonio Gomez-Pedrero
Feb 11, 2021·The Journal of Physical Chemistry. B·Balaka MondalGovardhan Reddy
Feb 12, 2021·Chembiochem : a European Journal of Chemical Biology·Megan A RochaRachel W Martin
Jun 8, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shu-Shun HsuehSteven S-S Wang
Jun 15, 2019·The Journal of Physical Chemistry. B·Rodrigo Aguayo-OrtizLaura Dominguez
Nov 21, 2019·Journal of Chemical Information and Modeling·Rodrigo Aguayo-Ortiz, Laura Dominguez
Jun 21, 2011·Journal of Molecular Biology·Huaying ZhaoPeter Schuck

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved