Partially reduced graphene oxide as highly efficient DNA nanoprobe

Biosensors & Bioelectronics
Yan-Hong WangWei Chen

Abstract

This work investigates the effect of reduction degree on graphene oxide (GO)-DNA interaction and the fluorescence quenching mechanism. Partial reduced graphene oxide (pRGO), which maintains well water-dispersibility, is synthesized using a mild reduction method by incubating GO suspension under alkaline condition at room temperature. The fluorescence quenching enhances with the restoration degree of sp(2) carbon bonds and follows the static quenching mechanism. The binding constant values imply that pRGO has much stronger affinity with ssDNA than GO. Utilizing this highly efficient nanoprobe, a universal sensing strategy is proposed for homogeneous detection of DNA. Compared with the reported GO-based DNA, this present strategy has obvious advantages such as requirement of low nanoprobe dosage, significantly reduced background, fast fluorescence quenching, and improved sensitivity. Even without any amplification process, the limit of detection can reach as low as 50 pM.

References

Oct 23, 2004·Science·K S NovoselovA A Firsov
Aug 21, 2009·ACS Nano·Hui-Lin GuoXing-Hua Xia
Dec 22, 2009·Chemical Society Reviews·Daniel R DreyerRodney S Ruoff
Aug 25, 2010·ACS Nano·Daniela C MarcanoJames M Tour
Feb 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Marissa WuJuewen Liu
Feb 11, 2012·Small·Po-Jung Jimmy Huang, Juewen Liu

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Citations

Mar 3, 2017·ACS Applied Materials & Interfaces·Bedanga SapkotaMeni Wanunu
Feb 22, 2017·Physical Chemistry Chemical Physics : PCCP·Fabiana A GutierrezPaulo R Bueno
Sep 3, 2021·Analytical Methods : Advancing Methods and Applications·Yan QinBin Liu

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