Participation of Fos protein at the nucleus tractus solitarius in inhibitory modulation of baroreceptor reflex response in the rat

Brain Research
C D ShihJ Y Chan

Abstract

We investigated the physiologic role of Fos protein at the nucleus tractus solitarius (NTS) in the modulation of baroreceptor reflex (BRR) in adult, male Sprague-Dawley rats that were anesthetized and maintained with pentobarbital sodium (40 mg/kg, i.p., with 10 mg/kg/h i.v. infusion supplements). Repeated and scheduled activation of the baroreceptors by transient hypertension induced by i.v. administration of phenylephrine (2.5, 5.0 or 10.0 micrograms/kg) resulted in a significant increase in Fos-like immunoreactivity (Fos-LI), primarily in the caudal part of the NTS. This increase in Fos-LI in the barosensitive NTS neurons was appreciably reduced by bilateral microinjection into the caudal NTS of an antisense oligonucleotide (20 pmol, 20 nl) designed to target a region of the c-fos mRNA that flanks the initiation codon (5'-129 to 143-3'). The same treatment also discernibly enhanced the BRR response, but elicited no appreciable effect on systemic arterial pressure or heart rate. On the other hand, bilateral application to the NTS of the corresponding sense oligonucleotide (20 pmol, 20 nl) or an antisense cDNA (20 pmol, 20 nl) that targeted a different site of the c-.fos mRNA (5'-135 to 149-3') was ineffective. These results s...Continue Reading

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Citations

Feb 6, 2004·Progress in Neurobiology·R A L Dampney, J Horiuchi
Jan 8, 1999·Brain Research·H E GreenbergD A Ruggiero
Aug 30, 2000·Respiration Physiology·A L SicaS M Scharf
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Apr 25, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Rishi L KhanJames S Schwaber
Dec 22, 1999·The Japanese Journal of Physiology·M MaedaM Nakai
Feb 20, 2013·Antioxidants & Redox Signaling·Samuel H H Chan, Julie Y H Chan
Feb 7, 2017·Journal of Applied Toxicology : JAT·Hoay Yan CheahLip Yong Chung
Dec 31, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ana Carolina Rodrigues Dias, Eduardo Colombari
Jul 28, 2012·Journal of Applied Physiology·Samuel H H Chan, Julie Y H Chan
Mar 29, 2014·Genome Research·James ParkRajanikanth Vadigepalli

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