Participation of mitochondrial metabolism in photorespiration. Reconstituted system of peroxisomes and mitochondria from spinach leaves

Plant Physiology
A S RaghavendraH W Heldt

Abstract

In this study the interplay of mitochondria and peroxisomes in photorespiration was simulated in a reconstituted system of isolated mitochondria and peroxisomes from spinach (Spinacia oleracea L.) leaves. The mitochondria oxidizing glycine produced serine, which was reduced in the peroxisomes to glycerate. The required reducing equivalents were provided by the mitochondria via the malate-oxaloacetate (OAA) shuttle, in which OAA was reduced in the mitochondrial matrix by NADH generated during glycine oxidation. The rate of peroxisomal glycerate formation, as compared with peroxisomal protein, resembled the corresponding rate required during leaf photosynthesis under ambient conditions. When the reconstituted system produced glycerate at this rate, the malate-to-OAA ratio was in equilibrium with a ratio of NADH/NAD of 8. 8 x 10(-3). This low ratio is in the same range as the ratio of NADH/NAD in the cytosol of mesophyll cells of intact illuminated spinach leaves, as we had estimated earlier. This result demonstrates that in the photorespiratory cycle a transfer of redox equivalents from the mitochondria to peroxisomes, as postulated from separate experiments with isolated mitochondria and peroxisomes, can indeed operate under con...Continue Reading

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Citations

Nov 11, 2003·Trends in Plant Science·Agepati S Raghavendra, Kollipara Padmasree
Jan 12, 2001·FEMS Microbiology Reviews·B M BakkerJ T Pronk
Jun 22, 1999·Current Opinion in Plant Biology·R Douce, M Neuburger
Sep 7, 2011·Biochimica Et Biophysica Acta·Juri DubininFrank Colditz
May 14, 2014·International Journal of Molecular Sciences·Daniela TronoDonato Pastore
Sep 1, 2005·Biochemical and Biophysical Research Communications·Lidia de BariSalvatore Passarella
Apr 10, 2007·Biochimica Et Biophysica Acta·Lidia de BariSalvatore Passarella
Jun 17, 2008·Annals of Botany·Owen K Atkin, David Macherel
Jan 1, 2010·The Arabidopsis Book·Christoph PeterhanselFritz Kreuzaler
Oct 30, 2016·BMC Systems Biology·Veronique BeckersChristoph Wittmann
Mar 9, 1999·Plant Physiology·I HanningH W Heldt
Sep 5, 2006·Plant Physiology·Daisuke IgarashiChieko Ohsumi

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