Participation of PLA2 and PLC in DhL-induced activation of Rhinella arenarum oocytes

Zygote : the Biology of Gametes and Early Embryos
J Zapata-MartínezG Sánchez-Toranzo

Abstract

Rhinella arenarum oocytes can be artificially activated, a process known as parthenogenesis, by a sesquiterpenic lactone of the guaianolide group, dehydroleucodine (DhL). Transient increases in the concentration of cytosolic Ca2+ are essential to trigger egg activation events. In this sense, the 1-4-5 inositol triphosphate receptors (IP3R) seem to be involved in the Ca2+ transient release induced by DhL in this species. We analyzed the involvement of phosphoinositide metabolism, especially the participation of phospholipase A2 (PLA2) and phospholipase C (PLC) in DhL-induced activation. Different doses of quinacrine, aristolochic acid (ATA) (PLA2 inhibitors) or neomycin, an antibiotic that binds to PIP2, thus preventing its hydrolysis, were used in mature Rhinella arenarum oocytes. In order to assay the participation of PI-PLC and PC- PLC we used U73122, a competitive inhibitor of PI-PLC dependent events and D609, an inhibitor of PC-PLC. We found that PLA2 inhibits quinacrine more effectively than ATA. This difference could be explained by the fact that quinacrine is not a specific inhibitor for PLA2 while ATA is specific for this enzyme. With respect to the participation of PLC, a higher decrease in oocyte activation was detect...Continue Reading

References

Feb 1, 1976·Biochemical Pharmacology·R J Flower, G J Blackwell
Jun 5, 1992·European Journal of Pharmacology·M YamadaE Richelson
Jun 26, 1992·Journal of Medicinal Chemistry·O S GiordanoG H Wendel
Jul 1, 1990·Journal of Natural Products·O S GiordanoT Guardia
Sep 21, 1989·Nature·M J Berridge, R F Irvine
Feb 1, 1988·The Biochemical Journal·N S GeeJ K Shute
Sep 30, 1993·Nature·M J Berridge
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·J D ClarkL L Lin
Aug 18, 2001·Zygote : the Biology of Gametes and Early Embryos·J OterinoM I Bühler
Apr 30, 2002·Developmental Biology·Linda L RunftLisa M Mehlmann
Sep 25, 2003·Biochemical Society Transactions·G HaletJ Carroll
Apr 15, 2004·Developmental Biology·Linda L RunftKathy R Foltz
Aug 19, 2004·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Qian ChuSuming Zhang
Oct 19, 2006·Cell Calcium·Michael Whitaker
Jun 26, 2008·Journal of Lipid Research·Douglas W PetcoffBradley J Stith
Oct 1, 2010·Zygote : the Biology of Gametes and Early Embryos·M T AjmatM I Bühler
Feb 4, 2012·Zygote : the Biology of Gametes and Early Embryos·M T AjmatM I Bühler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.