Participation of reactive oxygen species in phototoxicity induced by quinolone antibacterial agents

Archives of Biochemistry and Biophysics
Naoki UmezawaT Nagano

Abstract

To elucidate the mechanism of phototoxicity induced as a side effect by some of the new quinolone antibiotics, we studied sparfloxacin (SPFX), lomefloxacin, enoxacin, ofloxacin, and ciprofloxacin. We first examined the photosensitized formation of reactive oxygen species such as singlet oxygen (1O2) and superoxide anion (O2-) mediated by the new quinolones. Although a large number of studies have been reported, there is no direct evidence that these drugs generate reactive oxygen species. We employed a near-infrared emission spectrometer to detect 1O2-specific emission (1268 nm), and the nitroblue tetrazolium reduction method to detect O2-. All the quinolones investigated in this study were found to produce 1O2. Four drugs, but not SPFX, produced O2-. We also examined photodynamic DNA strand-breaking activity as a possible mechanism to explain the participation of reactive oxygen species in the phototoxicity of the drugs. All the drugs exhibited photodynamic DNA strand-breaking activity. The inhibitory effect of scavengers of reactive oxygen species indicated that the main active species was 1O2. The DNA strand-breaking activity was correlated not with the 1O2-forming ability, but with the affinity of the drugs for DNA. This re...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G PalùM Palumbo
Nov 1, 1991·Toxicology and Applied Pharmacology·D G RobertsonB Song
Aug 1, 1991·Photochemistry and Photobiology·T ArtusoN Paillous
Dec 1, 1990·Toxicology Letters·N WagaiK Tawara
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L L Shen, A G Pernet
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T Hasan, A U Khan
May 1, 1988·Photochemistry and Photobiology·P Dayhaw-Barker, T G Truscott
Jan 1, 1984·Photochemistry and Photobiology·D E MooreH Yip
May 1, 1995·Journal of Toxicology and Environmental Health·S TakayamaH Shimada
May 1, 1994·Biological & Pharmaceutical Bulletin·Y IwamotoY Yanagihara
Apr 1, 1994·The Journal of Antimicrobial Chemotherapy·J M Domagala
Apr 1, 1994·Journal of Dermatological Science·T HorioM Harada
Dec 1, 1993·Biological & Pharmaceutical Bulletin·Y IwamotoY Yanagihara

❮ Previous
Next ❯

Citations

Feb 24, 2006·Antimicrobial Agents and Chemotherapy·M GoswamiN Jawali
Oct 4, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tetsuo Nagano
Apr 8, 2006·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Eva MiadokováPeter Rauko
Jan 27, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Milan NagyJuraj Krajcovic
Jan 31, 2012·Drug and Chemical Toxicology·Rina TripathiPankaj Tripathi
Oct 30, 2013·Drug and Chemical Toxicology·Rina TripathiChhagan N Patel
Aug 24, 2010·Journal of Photochemistry and Photobiology. B, Biology·Maria Consuelo CuquerellaGuido de Guidi
Dec 10, 2015·Current Therapeutic Research, Clinical and Experimental·Majed M MasadehSayer I Al-Azzam
Sep 5, 2008·The Journal of Investigative Dermatology·Melanie StruweUlla Plappert-Helbig
Mar 27, 2009·Photochemistry and Photobiology·Virginie Lhiaubet-ValletMiguel Angel Miranda
Jun 11, 2009·Photochemistry and Photobiology·Fernando LorenzoNorman S Allen
Nov 30, 2005·Photodermatology, Photoimmunology & Photomedicine·Katsuhide SayamaMasako Sasaki
Sep 1, 2007·Clinical & Experimental Ophthalmology·Andrew M Thompson
Feb 11, 2012·Photochemistry and Photobiology·Yancheng LiuWenfeng Wang
Jul 21, 2011·Photochemistry and Photobiology·Guido de GuidiAlfio Catalfo
Aug 13, 2013·Photochemistry and Photobiology·Anna PolishchukVladimir Karasev
Oct 13, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nathalie DijouxPierre-Jacques Ferret
Dec 7, 2013·Journal of Medical Microbiology·Manish GoswamiNarendra Jawali
May 1, 1999·Toxicology and Applied Pharmacology·S J BuleraF A de la Iglesia
Oct 19, 2013·Biochimica Et Biophysica Acta·Igor MeerovichJean-Philippe Pellois
Sep 7, 2007·Cell·Michael A KohanskiJames J Collins
Jun 1, 2016·Drug and Chemical Toxicology·Vedat ŞekeroğluZülal Atlı Şekeroğlu
Dec 8, 2004·Toxicology and Applied Pharmacology·Grigorij KoganJuraj Krajcovic
Oct 4, 2016·Environmental Science and Pollution Research International·Jakub Trawiński, Robert Skibiński
Jan 8, 2005·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·L KrizkováJ Krajcovic
Aug 22, 2008·Human & Experimental Toxicology·S RampalN Sood
Sep 16, 2000·Photochemistry and Photobiology·S Navaratnam, J Claridge
Feb 19, 2002·Chemical & Pharmaceutical Bulletin·Tetsuya ArakiHiroaki Kitaoka
Jan 2, 2008·Phytotherapy Research : PTR·Lívia KrizkováJuraj Krajcovic
Feb 22, 2012·Current Microbiology·Majed M MasadehAshraf I Shaweesh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.