Particle-induced myeloperoxidase release in serially diluted whole blood quantifies the number and the phagocytic activity of blood neutrophils and opsonization capacity of plasma

Luminescence : the Journal of Biological and Chemical Luminescence
Esa-Matti Lilius, Jari Timo Juhani Nuutila

Abstract

Luminol-amplified chemiluminescence (CL) from phagocytes has previously been shown to be almost completely dependent on the release of myeloperoxidase (MPO) from azurophilic granules. We measured the luminol-amplified chemiluminescence response (WBCL) by using serially diluted whole blood. In these experiments, non-opsonized and serum-opsonized zymosan (NWBCL and OWBCL, respectively) were used concurrently as phagocytosable particles. We found two whole-blood dilution ranges with clinical significance: first, <0.04% of whole blood in the reaction volume, where MPO released by the zymosan-activated leukocyte population came almost totally from neutrophils and the OWBCL response could be exploited as a measure of a neutrophil count in a given blood specimen, despite the pathophysiological state of the host. In contrast, the NWBCL response was two-fold in blood samples from bacterial infection patients compared to those of controls and patients with viral infection, suggesting the use of NWBCL for the differential diagnosis of bacterial infections from viral infections; second, 0.16-1.2% of whole blood in the reaction volume, where the opsonization capacity of plasma (OC(50)) can be determined. We also found that at whole blood co...Continue Reading

References

Jul 7, 1978·Biochimica Et Biophysica Acta·A BosD Roos
Jul 1, 1976·The Journal of Clinical Investigation·H Rosen, S J Klebanoff
Dec 1, 1992·Experientia·E M Lilius, P Marnila
Jan 1, 1991·The Journal of Allergy and Clinical Immunology·M CarlsonP Venge
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·C DahlgrenK Orselius
Jan 1, 1989·Scandinavian Journal of Infectious Diseases·K PauksensP Venge
Dec 1, 1987·Journal of General Microbiology·S W EdwardsC A Hart
Apr 1, 1989·American Journal of Clinical Pathology·A TrulsonP Venge
May 2, 1986·Biochimica Et Biophysica Acta·O StendahlK E Magnusson
Jun 1, 1987·The Journal of Trauma·Z Y Sheng, Y L Tung
Dec 1, 1983·American Journal of Hematology·D Del PrincipeM L Scarpati
Jan 1, 1981·International Archives of Allergy and Applied Immunology·B D Cheson, S E Morris
May 1, 1995·Free Radical Biology & Medicine·V L van AntwerpenR Anderson
Nov 1, 1994·The Journal of Allergy and Clinical Immunology·S A Nordman, P W Nyberg
Aug 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Uehara, N Sato
Jul 1, 1993·The Journal of Infectious Diseases·D W BrarR A Proctor
Oct 1, 1995·Diabetic Medicine : a Journal of the British Diabetic Association·S J GallacherA C MacCuish
Jun 15, 2000·The European Respiratory Journal·S F van Eeden, J C Hogg
Mar 14, 2001·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·P A KorhonenA R Pösö
Apr 11, 2001·The Journal of Allergy and Clinical Immunology·J MonteseirínF Sobrino
Nov 22, 2001·JAMA : the Journal of the American Medical Association·R ZhangS L Hazen
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Massimo ConeseRuggiero Fumarulo
Apr 13, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jari Nuutila, Esa-Matti Lilius

❮ Previous
Next ❯

Citations

Apr 16, 2013·Veterinary Immunology and Immunopathology·Maaria KilpiEsa-Matti Lilius
Dec 1, 2015·Journal of Thermal Biology·Pertti Marnila, Esa-Matti Lilius
Jun 30, 2010·Luminescence : the Journal of Biological and Chemical Luminescence·Gorazd TompaPertti Marnila
Dec 9, 2014·Toxicology Letters·Martina JonssonKimmo Peltonen
Aug 15, 2017·Frontiers in Immunology·Liisa K VilénEsa-Matti Lilius

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Related Papers

Developmental and Comparative Immunology
Sami NikoskelainenEsa-Matti Lilius
Luminescence : the Journal of Biological and Chemical Luminescence
Martina Pavelkova, Lukas Kubala
Developmental and Comparative Immunology
Sami NikoskelainenEsa-Matti Lilius
Comparative Immunology, Microbiology and Infectious Diseases
M E CollazosE Ortega
Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
D H RohrG Young
© 2021 Meta ULC. All rights reserved