PMID: 8614644Apr 1, 1996Paper

Particle-mediated delivery of recombinant expression vectors to rabbit skin induces high-titered polyclonal antisera (and circumvents purification of a protein immunogen)

Nucleic Acids Research
P SundaramJ L Brandsma

Abstract

Polyclonal antibodies were generated in rabbits by delivery to skin of gold particles coated with mammalian expression vectors encoding a cytoplasmic (beta-galactosidase) or a nuclear (L1 capsid of cottontail rabbit papillomavirus) protein. One primary and one booster immunization of 30 micrograms DNA per rabbit yielded specific antisera with titers from 1:24 000 to 1:120 000 in each of eight rabbits, as detected by ELISA and Western blot analysis. Genetic immunization requires relatively small amounts of DNA, eliminates the need to purify the protein immunogen, and does not require irritating adjuvants.

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Citations

Apr 1, 1997·Molecular Biotechnology·P A Furth
Feb 28, 2001·Journal of Immunological Methods·P S ChowdhuryI Pastan
Feb 12, 2000·Journal of Immunological Methods·H GårdsvollG Høyer-Hansen
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Jul 17, 2009·Physical Chemistry Chemical Physics : PCCP·Wei-Chun LinJing-Jong Shyue
Apr 30, 2017·Chemical Science·Lev A Dykman, Nikolai G Khlebtsov
Sep 23, 2003·Journal of Medical Primatology·Nicole A Doria-RoseNancy L Haigwood
May 20, 2006·Science·Nathaniel L RosiChad A Mirkin

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