Particle tracking using confocal microscopy to probe the microrheology in a phase-separating emulsion containing nonadsorbing polysaccharide

Langmuir : the ACS Journal of Surfaces and Colloids
Thomas MoschakisEric Dickinson

Abstract

Brownian diffusion of fluorescent microspheres (0.21, 0.5, and 0.89 microm diameter) in conjunction with confocal microscopy has been used to monitor the microrheology of phase-separated regions in a protein-stabilized oil-in-water emulsion containing various low concentrations of a nonadsorbing polysaccharide, xanthan gum. The sensitivity and reliability of the technique has been demonstrated in test experiments on (i) aqueous glycerol solutions and (ii) concentrated surfactant-stabilized emulsions (30-60 vol % oil, 1-2 wt % Tween 20). From particle tracking measurements on the caseinate-stabilized emulsions (30 vol % oil, 1.4 wt % sodium caseinate, pH 7) containing xanthan (0.03-0.07 wt %), the apparent viscosity in the oil-droplet-rich regions has been estimated to be up to 10(3) times higher than that in the phase-separated xanthan-rich regions. This means that our previously determined shape relaxation times for xanthan-containing blobs in the same systems can be attributed to the dominant viscoelasticity of the surrounding regions of concentrated oil droplets and not to the rheology of the xanthan-rich blobs themselves. These data provide clear and unequivocal evidence for the dominant role of the interconnected depletion...Continue Reading

References

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Mar 23, 2005·Journal of Colloid and Interface Science·Thomas MoschakisEric Dickinson

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Citations

Mar 28, 2008·Analytical Chemistry·Jennifer C GaddDaniel T Chiu
Apr 26, 2013·Journal of Agricultural and Food Chemistry·Xiaolin YaoJiang Lu
Dec 12, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Bo ShuangChristy F Landes
Jan 10, 2012·Nature Protocols·Pei-Hsun WuDenis Wirtz
Feb 14, 2013·Applied Physics Letters·Christopher J Rowlands, Peter T C So
Jun 29, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Tomohito Hanazawa, Brent S Murray
Mar 13, 2010·Journal of Colloid and Interface Science·Thomas MoschakisEric Dickinson
Jan 3, 2013·Journal of the Science of Food and Agriculture·Eric Dickinson
Jun 23, 2010·Advances in Colloid and Interface Science·Eric Dickinson
Mar 23, 2012·Journal of Colloid and Interface Science·Thomas MoschakisCostas G Biliaderis
Jun 23, 2016·Journal of Agricultural and Food Chemistry·Lin ChenLin Yu
Jan 27, 2017·Annual Review of Food Science and Technology·Nan YangYapeng Fang
Aug 3, 2018·Small·Tommy Garting, Anna Stradner
Jun 13, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Michael H G DuitsFrieder Mugele
Feb 15, 2020·Food & Function·Pui Yeu Phoon, Christiani Jeyakumar Henry
Jul 6, 2018·Soft Matter·Matthew D WehrmanKelly M Schultz
Jul 20, 2006·Soft Matter·Eric Dickinson
Sep 16, 2017·Soft Matter·Li-Chiun ChengPatrick S Doyle
Feb 10, 2021·BMC Bioinformatics·Ermanno CordelliGiulio Iannello
Dec 2, 2009·Biomacromolecules·Maria V TzoumakiCostas G Biliaderis
Jan 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Hassan FiroozmandEric Dickinson
Feb 22, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Jes C KnudsenLeif H Skibsted
May 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Kavindya K Senanayake, Ashis Mukhopadhyay
Mar 21, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Jian Wu, Lenore L Dai

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