Particulate matter 2.5 induced arrhythmogenesis mediated by TRPC3 in human induced pluripotent stem cell-derived cardiomyocytes

Archives of Toxicology
Cheng CaiMinglong Chen

Abstract

Particulate matter (PM) is one of the most important environmental issues worldwide, which is associated with not only pulmonary but also cardiovascular diseases. However, the underlying biological mechanisms of PM related cardiovascular dysfunction remained poorly defined, especially mediated by the pathway of direct impact on vascular and heart. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) provide an ideal platform for studying PM-exposed cellular diseases model in vitro. Here, we investigated the direct effects of particulate matter 2.5 (PM2.5) on hiPSC-CMs and the potential mechanism at non-cytotoxic concentrations. Cell viability, contraction amplitude and spontaneous beating rate of iPSC-CMs after direct exposure to PM2.5 showed that the concentration of lower than 100 µg/ml would not lead to cytotoxic effects. Calcium-mediated optical mapping illustrated that there was a concentration-dependent reduction in quantification of calcium transient amplitude and an increase in the incidence of early after depolarizations due to PM2.5 treatment. Furthermore, there were dramatic dosage-dependent shortening in action potential duration and decrease in L-type calcium peak current density. The Ingenuity Pa...Continue Reading

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Citations

Jun 20, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Amina KunovacJohn M Hollander
Jul 7, 2020·Journal of Pharmacological and Toxicological Methods·Matthias GoßmannSonja Stoelzle-Feix
Sep 30, 2020·Journal of Molecular and Cellular Cardiology·Shuhui WangShengna Han
Apr 2, 2021·Reviews on Environmental Health·Liwei YangLin Ye

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Methods Mentioned

BETA
chips
flow cytometry

Software Mentioned

Zeiss Microscope Software ZEN
OriginPro
ImageJ
SigmaPlot
SPSS Statistics
IPA

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