Partition and permeation of dextran in polyacrylamide gel

Biophysical Journal
J C WilliamsS Eichholtz

Abstract

Partition of sized FITC-dextrans in polyacrylamide gel showed a relationship between Kav and solute radius as predicted by the theory of Ogston, which is based solely on geometry of the spaces. Permeability data for the same dextrans were fit to several theories, including those based on geometry and those based on hydrodynamic interactions, and the gel structure predicted by the partition and permeability data were compared. The Brinkman effective-medium model (based on hydrodynamic interactions and requiring a measure of the hydraulic conductivity of the matrix) gave the best fit of permeability data with the values for fiber radius (rf) and void volume of the gel (epsilon) that were obtained from the partition data. The models based on geometry and the hydrodynamic screening model of Cukier, using the rf and epsilon from partition data, all predicted higher rates of permeation than observed experimentally, while the effective-medium model with added term for steric interaction predicted lower permeation than that observed. The size of cylindrical pores appropriate for the partition data predicted higher rates of permeation than observed. These relative results were unaffected by the method of estimating void volume of the ge...Continue Reading

References

Oct 1, 1975·Analytical Biochemistry·R Rüchel, M D Brager
Aug 1, 1992·Journal of the American Society of Nephrology : JASN·J D OliverW H Deen
Feb 7, 1992·Journal of Chromatography·M MoussaouiP Wahl
Jul 1, 1980·Microvascular Research·F E Curry, C C Michel
Apr 1, 1995·Biophysical Journal·E M JohnsonW M Deen
Oct 1, 1994·Proceedings of the Society for Experimental Biology and Medicine·J C Williams
Jul 11, 1996·The American Journal of Physiology·R L RiversJ C Williams
Mar 1, 1996·Biophysical Journal·J Tong, J L Anderson
Feb 1, 1996·Biophysical Journal·E M JohnsonW M Deen
Jul 1, 1994·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·M A Katz, M LaMarche
Nov 1, 1996·Biophysical Journal·J D Jones, K Luby-Phelps
Jan 1, 1961·Biophysical Journal·M A LAUFFER

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Citations

Mar 3, 2004·Bioelectrochemistry·David A Zaharoff, Fan Yuan
Jul 19, 2008·Biomaterials·Shufang ZhangHanry Yu
Jun 8, 2012·Biotechnology and Bioengineering·Giovanni G GiobbeNicola Elvassore
Sep 27, 2005·Journal of Biotechnology·D A ZubtsovA S Zasedatelev
Jun 3, 2016·The Journal of Chemical Physics·D WagnerH E Hermes
May 30, 2017·ACS Applied Materials & Interfaces·Lydia KisleyDeborah E Leckband
Dec 9, 2003·Applied Spectroscopy·Sungjong Kwak, Michel Lafleur
Aug 27, 2004·Biotechnology and Bioengineering·Dick van RoosmalenDaniel Blankschtein
Apr 30, 2005·Electrophoresis·Upma Sharma, Jeffrey D Carbeck
Mar 12, 2005·Annals of Botany·Timothy E Proseus, John S Boyer
Dec 3, 2020·APL Bioengineering·Alden C Moss, Amy E Herr
Jan 21, 2021·Physical Review. E·Frank J AangenendtHans M Wyss
Jan 21, 2021·Physical Review. E·Melle T J J M PunterBela M Mulder
Nov 5, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Sujit Basak, Krishnananda Chattopadhyay
Nov 23, 2019·Analytical Chemistry·Alison SuAmy E Herr
Dec 31, 2019·Biomacromolecules·Eleonóra Parelius JonášováBjørn Torger Stokke

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