Partition of amphiphilic molecules to lipid bilayers by isothermal titration calorimetry

Analytical Biochemistry
Maria João MorenoAdrian Velazquez-Campoy

Abstract

The partition of the amphiphile sodium dodecyl sulfate (SDS) between an aqueous solution and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayer was followed by isothermal titration calorimetry (ITC) as a function of the total concentration of SDS. It was found that the obtained partition coefficient is strongly affected by the ligand concentration, even after correction for the charge imposed in the bilayer by the bound SDS. The partition coefficient decreased as the total concentration of SDS increased, with this effect being significant for local concentrations of SDS in the lipid bilayer above 5 molar%. At those high local concentrations, the properties of the lipid bilayer are strongly affected, leading to nonideal behavior and concentration-dependent apparent partition coefficients. It is shown that with the modern ITC instruments available, the concentrations of SDS can be drastically reduced while maintaining a good signal-to-noise ratio. The intrinsic parameters of the interaction with unperturbed membranes can be obtained from the asymptotic behavior of the apparent parameters as a function of the ligand concentration for both nonionic and ionic solutes. A detailed analysis is performed, and a spreadshee...Continue Reading

References

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Citations

Aug 5, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Filipe M Coreta-GomesMaria João Moreno
Jan 4, 2012·Journal of Molecular Recognition : JMR·Rajesh GhaiBrett M Collins
Feb 24, 2019·The Journal of General Physiology·Ruchi KapoorOlaf S Andersen
Dec 16, 2010·Journal of Molecular Recognition : JMR·Robert J Falconer, Brett M Collins
Nov 26, 2010·The Journal of Physical Chemistry. B·R M S CardosoM J Moreno
Oct 13, 2010·The Journal of Physical Chemistry. B·Guangyue BaiMargarida Bastos
Feb 22, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaona JingHanne M Nielsen
Feb 3, 2012·Journal of the American Chemical Society·Patrícia T MartinsMaria João Moreno
Oct 18, 2017·ACS Omega·Jaime SameloMaria João Moreno
Jul 11, 2013·The Journal of Physical Chemistry. B·Ioan Turcu, Mihaela Mic

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