PMID: 20953Oct 17, 1977

Partition of catalase and its peroxidase activities in human red cell membrane: effect of ATP depletion

Biochimica Et Biophysica Acta
L M SnyderN L Fortier

Abstract

Partititon of catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase EC 1.11.1.6) and peroxidase (donor:hydrogen-peroxide oxidoreductase EC 1.11.1.7) activities between the red cell membrane and the cytosol were studied under various experimental conditions. A small but significant amount of catalase (1.6%) was retained on human red cell membranes prepared by hemolysing washed red cells with 30 volumes of 10 mM Tris buffer, pH 7.4. Membrane -bound catalase had a relatively higher peroxidase activity than the soluble enzyme fraction. Polyacrylamide gel electrophoresis in sodium dodecyl sulfate of the solubilized membranes demonstrated catalase to be a single band with a molecular weight of 60 000. Membranes prepared from adenosine triphosphate-depleted red cells depicted a two to three-fold increase in catalase activity, as well as an increase in 60 000 molecular weight band on polyacrylamide gel electrophoresis. The extra amount of retained catalase was a less efficient peroxidase than found in fresh membranes. The binding of catalase to ATP-depleted red cell membranes was dependent upon both pH and hemolysing ratio. Red cells incubated at pH 7.1 demonstrated a decrease in bound catalase, as did membranes prepared from re...Continue Reading

References

Mar 1, 1974·Analytical Biochemistry·E M Gregory, I Fridovich
Jul 1, 1974·The Journal of Cell Biology·T L Steck
Sep 15, 1972·Biochimica Et Biophysica Acta·P Nicholis
Jun 1, 1962·The Journal of Clinical Investigation·M A HENNESSEYB W GABRIO
Apr 9, 1962·Biochimica Et Biophysica Acta·P NICHOLLS

Citations

Jul 1, 1980·Journal of the Neurological Sciences·A ZanellaG Scarlato
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S PontremoliB L Horecker
Apr 13, 2005·Medical Hypotheses·George E Parris
Jun 15, 1982·Biochemical and Biophysical Research Communications·S PontremoliB L Horecker
Jan 1, 1979·Journal of Supramolecular Structure·J Palek, S C Liu

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Adenosine Triphosphate, Chromium Ammonium Salt
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