PMID: 4578150Jul 1, 1973

Partition of Salmonella typhimurium in a two-polymer acqueous phase system in relation to liability to phagocytosis

Infection and Immunity
O StendahlM Edebo

Abstract

Analysis of the partition in a two-polymer phase system of Salmonella typhimurium R mutants with different susceptibility to phagocytosis distinguished between the mutants. Close to 80% of the R mutants least resistant to phagocytosis accumulated in the dextran-rich phase, whereas more phagocytosis-resistant mutants showed higher affinity for the interface and the polyethylene glycol-rich top phase. By growing the uridine diphosphate (pyro)-gal-4-epimeraseless mutant LT2-M1 in the presence of d-galactose for different periods of time, two well-defined peaks were obtained in counter-current distribution analysis with an aqueous two-polymer phase system revealing a heterogeneous population in the culture. One peak was located at a site characteristic of R bacteria, the other at the site of S bacteria. As growth proceeded, more bacteria transferred from the R-type to the S-type peak. Within each peak, a gradual increase in resistance to phagocytosis by rabbit polymorphonuclear cells occurred with increasing length of growth in d-galactose.

Citations

Jan 1, 1975·Immunological Communications·L EdeboC Tagesson
Aug 1, 1982·Journal of Cellular Physiology·O StendahlJ Hed
Sep 1, 1993·The Journal of Applied Bacteriology·C W Blackburn
Oct 1, 1977·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·O StendahlS Hjertén
Oct 1, 1990·Journal of Biomaterials Applications·M C van LoosdrechtA J Zehnder
Apr 1, 1979·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·O Stendahl, B Normann
Jan 1, 1980·Scandinavian Journal of Infectious Diseases. Supplementum
Dec 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A Walan, E Kihlström
Apr 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·J HuixiuL Edebo
Feb 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·H MiörnerG Kronvall
Apr 1, 1979·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·O StendahlL Edebo
Oct 1, 1977·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·L PerersC Tagesson
Apr 1, 1978·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·K E MagnussonL Edebo
Apr 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·L OhmanO Stendahl

Related Concepts

Countercurrent Chromatography
Science of Microbial Genetics
Phagocytosis
Salmonella Phages
Salmonella typhimurium LT2

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.