Partitioning of Antibiotic Resistance Genes and Fecal Indicators Varies Intra and Inter-Storm during Combined Sewer Overflows

Frontiers in Microbiology
Alessia EramoNicole L Fahrenfeld

Abstract

Combined sewer overflows (CSOs) degrade water quality through the release of microbial contaminants in CSO effluent. Improved understanding of the partitioning of microbial contaminants onto settleable particles can provide insight into their fate in end-of-pipe treatment systems or following release during CSO events. Sampling was performed across the hydrograph for three storm events as well as during baseflow and wet weather in three surface waters impacted by CSO. qPCR was performed for select antibiotic resistance genes (ARG) and a marker gene for human fecal indicator organisms (BacHum) in samples processed the partitioning of microbial contaminants on settleable particles versus suspended in the aqueous phase. Amplicon sequencing was performed on both fractions of storm samples to further define the timing and partitioning of microbial contaminants released during CSO events. Samples collected at the CSO outfall exhibited microbial community signatures of wastewater at select time points early or late in the storm events. CSOs were found to be a source of ARG. In surrounding surface waters, sul1 was higher in samples from select locations during wet weather compared to baseflow. Otherwise, ARG concentrations were variabl...Continue Reading

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Citations

Jan 5, 2021·Water Environment Research : a Research Publication of the Water Environment Federation·Paige E Peters, Daniel H Zitomer
Mar 4, 2019·The Science of the Total Environment·Ayad AlmakkiPatricia Licznar-Fajardo
Jul 24, 2021·Environmental Science : Water Research & Technology·Eramo AlessiaN L Fahrenfeld

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Methods Mentioned

BETA
electrophoresis
amplicon sequencing

Software Mentioned

R Core Team
R
PRIMER
mothur MiSeq Standard Operating Procedure

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