Partitioning of mesophyll conductance for CO2 into intercellular and cellular components using carbon isotope composition of cuticles from opposite leaf sides

Photosynthesis Research
J ŠantrůčekJ Neuwirthová

Abstract

We suggest a new technique for estimating the relative drawdown of CO2 concentration (c) in the intercellular air space (IAS) across hypostomatous leaves (expressed as the ratio cd/cb, where the indexes d and b denote the adaxial and abaxial edges, respectively, of IAS), based on the carbon isotope composition (δ13C) of leaf cuticular membranes (CMs), cuticular waxes (WXs) or epicuticular waxes (EWXs) isolated from opposite leaf sides. The relative drawdown in the intracellular liquid phase (i.e., the ratio cc/cbd, where cc and cbd stand for mean CO2 concentrations in chloroplasts and in the IAS), the fraction of intercellular resistance in the total mesophyll resistance (rIAS/rm), leaf thickness, and leaf mass per area (LMA) were also assessed. We show in a conceptual model that the upper (adaxial) side of a hypostomatous leaf should be enriched in 13C compared to the lower (abaxial) side. CM, WX, and/or EWX isolated from 40 hypostomatous C3 species were 13C depleted relative to bulk leaf tissue by 2.01-2.85‰. The difference in δ13C between the abaxial and adaxial leaf sides (δ13CAB - 13CAD, Δb-d), ranged from - 2.22 to + 0.71‰ (- 0.09 ± 0.54‰, mean ± SD) in CM and from - 7.95 to 0.89‰ (- 1.17 ± 1.40‰) in WX. In contrast, two ...Continue Reading

References

Dec 7, 2002·Progress in Lipid Research·L Kunst, A L Samuels
Jan 31, 2006·The New Phytologist·Tracy Lawson, James Morison
Nov 13, 2007·Plant, Cell & Environment·Jaume FlexasHipólito Medrano
Mar 17, 2009·Journal of Experimental Botany·Foteini HassiotouJohn R Evans
Apr 10, 2009·Journal of Experimental Botany·Thijs L PonsEnrico Brugnoli
Apr 28, 2009·Journal of Experimental Botany·John R EvansIchiro Terashima
Apr 28, 2009·Journal of Experimental Botany·Ulo NiinemetsCharles R Warren
Jul 27, 2010·Trends in Plant Science·Lukas Schreiber
Dec 17, 2011·Rapid Communications in Mass Spectrometry : RCM·Li GaoYongsong Huang
May 18, 2012·Plant, Cell & Environment·Danny TholenXin-Guang Zhu
Jul 17, 2012·Plant Science : an International Journal of Experimental Plant Biology·Jaume FlexasCharles R Warren
Jul 31, 2013·Plant Physiology·Trevor H Yeats, Jocelyn K C Rose
Aug 2, 2013·The New Phytologist·Lucas A CernusakGraham D Farquhar
Aug 14, 2015·Proceedings. Biological Sciences·Christopher D Muir
Mar 1, 1994·The New Phytologist·David F Parkhurst

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Citations

Jun 19, 2019·Photosynthesis Research·Nerea Ubierna, Lucas A Cernusak

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