Partitioning of N-ethylmaleimide-sensitive fusion (NSF) protein function in Drosophila melanogaster: dNSF1 is required in the nervous system, and dNSF2 is required in mesoderm.

Genetics
J A GolbyL Pallanck

Abstract

The N-ethylmaleimide-sensitive fusion protein (NSF) promotes the fusion of secretory vesicles with target membranes in both regulated and constitutive secretion. While it is thought that a single NSF may perform this function in many eukaryotes, previous work has shown that the Drosophila genome contains two distinct NSF genes, dNSF1 and dNSF2, raising the possibility that each plays a specific secretory role. To explore this possibility, we generated mutations in the dNSF2 gene and used these and novel dNSF1 loss-of-function mutations to analyze the temporal and spatial requirements and the degree of functional redundancy between dNSF1 and dNSF2. Results of this analysis indicate that dNSF1 function is required in the nervous system beginning at the adult stage of development and that dNSF2 function is required in mesoderm beginning at the first instar larval stage of development. Additional evidence suggests that dNSF1 and dNSF2 may play redundant roles during embryonic development and in the larval nervous system. Ectopic expression studies demonstrate that the dNSF1 and dNSF2 gene products can functionally substitute for one another. These results indicate that the Drosophila NSF proteins exhibit similar functional properti...Continue Reading

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Citations

Apr 19, 2005·Genetics·Matthew J LavioletteBryan A Stewart
Dec 2, 2006·Genesis : the Journal of Genetics and Development·Jean-Baptiste PeyreBryan A Stewart
May 25, 2005·The Journal of Comparative Neurology·Bryan A StewartRadhika Khorana
Aug 24, 2021·Frontiers in Molecular Neuroscience·Chad W Sauvola, J Troy Littleton
Apr 4, 2006·Current Biology : CB·Ian G WoodsWilliam S Talbot
Sep 24, 2002·Journal of Cell Science·Roland KissmehlJean Cohen
Apr 3, 2007·FEBS Letters·Chunxia ZhaoSidney W Whiteheart
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J T LittletonB Ganetzky
Jul 16, 2010·Experimental Cell Research·Rika NagaiMasamitsu Yamaguchi
Feb 15, 2011·Molecular and Cellular Neurosciences·Wenhua YuRichard W Ordway

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