PARVA promotes metastasis by modulating ILK signalling pathway in lung adenocarcinoma

PloS One
Ay-Huey HuangSung-Liang Yu

Abstract

α-parvin (PARVA) is known to be involved in the linkage of integrins, regulation of actin cytoskeleton dynamics and cell survival. However, the role that PARVA plays in cancer progression remains unclear. Here, using a lung cancer invasion cell line model and expression microarrays, we identify PARVA as a potential oncogene. The overexpression of PARVA increased cell invasion, colony-forming ability and endothelial cell tube formation. By contrast, knockdown of PARVA inhibited invasion and tube formation in vitro. Overexpression of PARVA also promoted tumorigenicity, angiogenesis and metastasis in in vivo mouse models. To explore the underlying mechanism, we compared the expression microarray profiles of PARVA-overexpressing cells with those of control cells to identify the PARVA-regulated signalling pathways. Pathway analysis showed that eight of the top 10 pathways are involved in invasion, angiogenesis and cell death. Next, to identify the direct downstream signalling pathway of PARVA, 371 significantly PARVA-altered genes were analysed further using a transcription factor target model. Seven of the top 10 PARVA-altered transcription factors shared a common upstream mediator, ILK. Lastly, we found that PARVA forms a complex ...Continue Reading

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Citations

Nov 8, 2015·Biochimica Et Biophysica Acta·Ji-Hye YoonHee-Sae Park
Feb 2, 2018·Nature Communications·Guermarie Velazquez-TorresMenashe Bar-Eli
May 8, 2019·Journal of Cellular Biochemistry·Yuandi XiangJuan Wu
Dec 4, 2020·Pharmaceuticals·Ilaria GuerrieroArturo Orlacchio
Mar 4, 2021·Biotechnology and Applied Biochemistry·Huiming YuDazhao Wang

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Datasets Mentioned

BETA
GSE50657

Methods Mentioned

BETA
PCR
Transfection
protein assay
immunoprecipitation
zymography
immunoprecipitation assay

Software Mentioned

JMP Genomics
MetaCore
Image J
PARVA
Excel
GeneGo
MetaXpress

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