Parvalbumin, a cross-reactive fish allergen, contains IgE-binding epitopes sensitive to periodate treatment and Ca2+ depletion

The Journal of Allergy and Clinical Immunology
A Bugajska-SchretterS Spitzauer

Abstract

Type I allergy to fish is a severe health problem in countries in which a large percentage of the population derive income from fishing. The aim of the study was to characterize cross-reactive IgE-binding components in six different fish species (cod, tuna, salmon, perch, carp, and eel). The effect of reducing extraction conditions, periodate treatment, and depletion of Ca2+ on binding of IgE to the allergens was investigated. Extracts were prepared under nonreducing and reducing conditions. IgE-binding components were characterized by IgE immunoblotting, and cross-reactive epitopes were studied by IgE-immunoblot inhibition experiments. To reveal calcium-sensitive or carbohydrate-containing epitopes, nitrocellulose-blotted extracts were exposed to ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) and periodate. Sera from all patients allergic to fish (n = 30) displayed IgE reactivity to parvalbumin, a 12 kd protein present in fish extracts from six different species. Reducing extraction conditions had no effect on IgE binding to parvalbumins, whereas periodate treatment and depletion of protein-bound calcium led to a substantial reduction of IgE binding. Parvalbumins from six different species contain...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1975·Scandinavian Journal of Immunology·S Elsayed, H Bennich
Nov 1, 1992·The Journal of Pediatrics·C PascualJ F Crespo
Mar 1, 1992·The Journal of Allergy and Clinical Immunology·J Bernhisel-BroadbentH A Sampson
Mar 1, 1991·Trends in Biochemical Sciences·C W Heizmann, W Hunziker
Dec 1, 1990·The Journal of Allergy and Clinical Immunology·M de MartinoA Vierucci
Apr 8, 1985·Journal of Immunological Methods·M P WoodwardR A Bloodgood
Sep 1, 1982·Acta paediatrica Scandinavica·M Kajosaari
Oct 1, 1996·Scandinavian Journal of Immunology·C D LindstrømS Elsayed
Nov 1, 1946·The Journal of Allergy·L TUFTV M HECK
Jan 1, 1992·International Archives of Allergy and Immunology·Susanne SeiberlerRudolf Valenta

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Citations

Feb 14, 2004·Methods : a Companion to Methods in Enzymology·Barbara Bohle, Stefan Vieths
Apr 7, 2006·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·UNKNOWN American College of Allergy, Asthma, & Immunology
Oct 30, 2003·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Soili Mäkinen-KiljunenElina Varjonen
Jan 5, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·A HelblingS B Lehrer
May 5, 1999·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·S YamadaE Zychlinsky
Jul 2, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·F HuriauxB Focant
Dec 22, 1999·Allergy·R AseroP Falagiani
Apr 12, 2001·Allergy·L K PoulsenC Bindslev-Jensen
Oct 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A LedesmaR Rodríguez
Nov 16, 2004·Critical Reviews in Food Science and Nutrition·E N Clare MillsPeter R Shewry
May 25, 2013·The Annals of Occupational Hygiene·Anna Dahlman-HöglundEva Andersson
Apr 17, 2001·Bioscience, Biotechnology, and Biochemistry·Y HamadaK Shiomi
Apr 25, 2000·Biochemical and Biophysical Research Communications·B TangV P Kurup
Dec 18, 2012·Clinical Reviews in Allergy & Immunology·Nicki Y H LeungPatrick S C Leung
Feb 27, 2013·Clinical Reviews in Allergy & Immunology·Michael F Sharp, Andreas L Lopata
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·A R LorenzS Vieths
Feb 16, 2016·Allergology International : Official Journal of the Japanese Society of Allergology·Yukihiro KobayashiNaoko Hamada-Sato
Jun 30, 2012·Cell Calcium·Sergei E PermyakovEugene A Permyakov
May 1, 2012·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Kamal D SrivastavaXiu-Min Li
Dec 30, 2015·Critical Reviews in Food Science and Nutrition·Telmo J R FernandesIsabel Mafra
Jul 6, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mariola A DietrichAndrzej Ciereszko
Aug 5, 2009·Cell Calcium·Sergei E PermyakovEugene A Permyakov
Oct 22, 2008·Biochimica Et Biophysica Acta·Sergei E PermyakovEugene A Permyakov
Feb 6, 2007·Immunology and Allergy Clinics of North America·Nicole WopfnerPeter Lackner
Feb 26, 2004·Allergy·F FerreiraA Mari
May 28, 2008·Molecular Nutrition & Food Research·Yan MaHeimo Breiteneder
Apr 15, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M Perez-GordoC Pastor-Vargas
Aug 5, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J Sastre
Jun 22, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A KuehnF Hentges
May 25, 2010·Journal of Food Science·Stef J KoppelmanSteve L Taylor
Apr 5, 2013·Molecular Nutrition & Food Research·Marina Perez-GordoHugh A Sampson
Nov 18, 2003·Allergy·V RodríguezB Bartolomé
Nov 26, 2009·Molecular Nutrition & Food Research·Ulrike GriesmeierHeimo Breiteneder
May 2, 2013·Biochimica Et Biophysica Acta·Yulia S LaptevaSergei E Permyakov
Mar 10, 2015·The Journal of Allergy and Clinical Immunology·Nikolaos DouladirisNikolaos G Papadopoulos
Apr 17, 2015·Journal of Agricultural and Food Chemistry·Harmen H J de JonghStef J Koppelman
Nov 1, 2006·Allergology International : Official Journal of the Japanese Society of Allergology·Harumi KoyamaAtsuo Urisu

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