Parvovirus B19 DNA in plasma pools and plasma derivatives

Vox Sanguinis
I SchmidtJohannes Löwer

Abstract

Human parvovirus B19 (B19) has been transmitted by various plasma-derived medicinal products. The aim of this study was to determine the frequency and the level of B19 DNA contamination in plasma pools destined for fractionation and in a broad range of plasma derivatives. In addition, removal of B19 DNA by the manufacturing process was investigated in cases where corresponding samples from plasma pool and product were available. Plasma pool samples and blood products were tested for B19 DNA by nested polymerase chain reaction (PCR), and the viral DNA content was determined by TaqMan quantitative PCR. Two-hundred and twenty two of 372 plasma pools for fractionation contained B19 DNA at concentrations of 10(2)-10(8) genome equivalents/ml (geq/ml). While approximately 65% of the DNA-positive plasma pools were only moderately contaminated (< 10(5) geq/ml), 35% contained > 10(6) geq/ml. High frequencies of contamination were detected in Factor VIII (79 of 91), prothrombin complex concentrates (38 of 43) and Factor IX (41 of 62), where the concentration of B19 DNA ranged between 102 and 107 geq/ml. A lower level of B19 DNA contamination was found in antithrombin III (five of 26 samples), in anti-D immunoglobulins (three of 37 samples...Continue Reading

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Citations

Dec 25, 2003·Transfusion·Marco H G M KoppelmanHans L Zaaijer
Aug 31, 2002·Vox Sanguinis·B Just, H Lefèvre
Oct 19, 2002·Transfusion·Johannes BlümelJohannes Löwer
Nov 8, 2002·Transfusion·Johannes BlümelAnna Maria Eis-Hübinger
Mar 6, 2003·Vox Sanguinis·Celso Bianco
Jan 19, 2006·Clinical Microbiology Reviews·M J EspyT F Smith
Sep 18, 2012·Journal of Translational Medicine·Wei ZhangWuping Li
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Jun 7, 2005·Transfusion·Chuan-ging WuMei-ying W Yu
Apr 3, 2003·Vox Sanguinis·M YunokiK Ikuta
Sep 26, 2003·Transfusion·Irina Knezevic-Maramica, Margot S Kruskall
Aug 8, 2007·Transfusion Medicine·A Parsyan, D Candotti
Oct 3, 2012·British Journal of Haematology·Päivi NorjaMike Makris
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Nov 4, 2016·Clinical Microbiology Reviews·Jianming QiuNeal S Young
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