Parylene-A coated microplate for covalent immobilization of proteins and peptides

Journal of Immunological Methods
Byung-Jin JeonJae-Chul Pyun

Abstract

Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400-500 nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules.

References

Jan 22, 2000·Arthritis and Rheumatism·G A SchellekensW J van Venrooij
May 5, 2000·Biomolecular Engineering·H Schetters
Dec 2, 2006·Colloids and Surfaces. B, Biointerfaces·Tatsuro GodaKazuhiko Ishihara

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Citations

Aug 16, 2011·Analytical and Bioanalytical Chemistry·Yaodong ZhangTingting Hei
Sep 6, 2011·ACS Combinatorial Science·Meshude AkbulutSergey A Piletsky
Sep 29, 2011·Biosensors & Bioelectronics·Hyuk KoJae-Chul Pyun
Sep 7, 2012·Biosensors & Bioelectronics·Ga-Yeon LeeJae-Chul Pyun
Aug 31, 2014·Analytica Chimica Acta·Ha-Wook JungJae-Chul Pyun

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