PAS-C platelets contain less plasma protein, lower anti-A and anti-B titers, and decreased HLA antibody specificities compared to plasma platelets

Transfusion
Stuart P WeisbergClaudia S Cohn

Abstract

Platelets (PLTs) collected and stored in PLT additive solution Intersol (PAS-C) are presumed to reduce recipient exposure to donor plasma components; however, the effects of PAS-C on PLT supernatant composition are poorly defined. Therefore, we compared the total protein concentration, isohemagglutinin titers, and HLA antibodies in supernatants of PAS-C PLTs to plasma PLTs. Apheresis PLTs from group O blood donors were collected into either 100% donor plasma (n = 50) or a mixture of 65% PAS-C/35% donor plasma (n = 50). Within 12 hours of collection, samples of the PLT supernatant were frozen and stored. PLT supernatants were assayed for total protein concentration and anti-A and anti-B titers and screened for HLA antibodies. Samples positive for HLA antibodies were further tested using single-antigen bead assays to determine antibody strength and specificity. Supernatants of PAS-C PLTs had significantly lower total protein concentration and anti-A and anti-B titers compared to plasma PLTs. There was no significant difference in the number of HLA antibody screen-positive PAS-C (3/50) compared to plasma PLT supernatants (2/50); however, the HLA antibody screen-positive supernatants of PAS-C PLTs had fewer HLA specificities (2) co...Continue Reading

References

Mar 8, 2007·British Journal of Haematology·Jürgen Bux, Ulrich J H Sachs
Oct 24, 2008·Transplantation·Luis E Morales-BuenrostroJosefina Alberú
Feb 22, 2011·Transfusion·Danielle M CarrickUNKNOWN National Heart, Lung, and Blood Institute Retrovirus Epidemiology Donor Study-II
Apr 17, 2014·Transfusion·Claudia S CohnW Andrew Heaton
Jul 30, 2014·International Journal of Immunogenetics·T RobertsR A Bray

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Citations

Jan 18, 2020·Transfusion·Gizem TumerUNKNOWN Biomedical Excellence for Safer Transfusion (BEST) Collaborative
Feb 15, 2020·Transfusion·Nancy M Dunbar, Mark H Yazer
Mar 12, 2020·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Gayathiri K ChellaiyaSangeetha K Nayanar
Nov 10, 2020·Transfusion·Gaurav K GuptaChristopher A Tormey

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