Pasiflora proteins are novel core components of the septate junction

Development
Myrto DeligiannakiUlrike Gaul

Abstract

Epithelial sheets play essential roles as selective barriers insulating the body from the environment and establishing distinct chemical compartments within it. In invertebrate epithelia, septate junctions (SJs) consist of large multi-protein complexes that localize at the apicolateral membrane and mediate barrier function. Here, we report the identification of two novel SJ components, Pasiflora1 and Pasiflora2, through a genome-wide glial RNAi screen in Drosophila. Pasiflora mutants show permeable blood-brain and tracheal barriers, overelongated tracheal tubes and mislocalization of SJ proteins. Consistent with the observed phenotypes, the genes are co-expressed in embryonic epithelia and glia and are required cell-autonomously to exert their function. Pasiflora1 and Pasiflora2 belong to a previously uncharacterized family of tetraspan membrane proteins conserved across the protostome-deuterostome divide. Both proteins localize at SJs and their apicolateral membrane accumulation depends on other complex components. In fluorescence recovery after photobleaching experiments we demonstrate that pasiflora proteins are core SJ components as they are required for complex formation and exhibit restricted mobility within the membrane ...Continue Reading

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Citations

Feb 26, 2016·Biophysical Journal·Ferdinand GreissDieter Braun
Feb 6, 2016·Journal of Cell Science·Yasushi IzumiMikio Furuse
Jul 8, 2021·Molecular Ecology·Gabriela Montejo-KovacevichNicola J Nadeau

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Methods Mentioned

BETA
electron microscopy
transgenic
PCR
in vitro transcription

Software Mentioned

Definiens
BLAST
ClustalW
ZEN
PSI
Vector NTI

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