Passaging protocols for mammalian neural stem cells in suspension bioreactors

Biotechnology Progress
A SenL A Behie

Abstract

Mammalian neural stem cells (NSC) offer great promise as therapeutic agents for the treatment of central nervous system disorders. As a consequence of the large numbers of cells that will be needed for drug testing and transplantation studies, it is necessary to develop protocols for the large-scale expansion of mammalian NSC. Neural stem cells and early progenitor cells can be expanded in vitro as aggregates in controlled bioreactors using carefully designed media. The first objective of this study was to determine if it is possible to maintain a population of murine neural stem and progenitor cells as aggregates in suspension culture bioreactors over extended periods of time. We discovered that serial passaging of a mixture of aggregates sizes resulted in high viabilities, high viable cell densities, and good control of aggregate diameter. When the NSC aggregates were serially subcultured three times without mechanical dissociation, a total multiplication ratio of 2.9 x 10(3) was achieved over a period of 12 days, whereas the aggregate size was controlled (mean diameter less than 150 microm) below levels at which necrosis would occur. Moreover, cell densities of 1.0 x 10(6) cells/mL were repeatedly achieved in batch culture w...Continue Reading

Citations

Jun 14, 2003·Medical & Biological Engineering & Computing·M S KallosL A Behie
Nov 21, 2007·Bioprocess and Biosystems Engineering·Yuen Ling Ng, Howard A Chase
Sep 25, 2003·Cell·Antonio P BeltramiPiero Anversa
Apr 12, 2002·Brain Research. Developmental Brain Research·Arindom SenLeo A Behie
Feb 1, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Chou Chai, Kam W Leong
Nov 14, 2008·Stem Cells and Development·Galia YirmeJoseph Itskovitz-Eldor
Jun 4, 2005·Biotechnology Progress·Benjamin S YounJohn A Hassell
May 25, 2004·Expert Opinion on Biological Therapy·Julie Audet
Nov 6, 2004·Brain Research. Developmental Brain Research·Hsingchi J LinWu Ma
Apr 29, 2015·International Journal of Radiation Biology·Hyeon Soo EomUhee Jung
Jul 29, 2017·Journal of Tissue Engineering and Regenerative Medicine·Madiha KhurshidArindom Sen
Sep 29, 2006·Biotechnology and Bioengineering·Elsa AbranchesJoaquim M S Cabral
Jan 20, 2007·Journal of Biomedical Materials Research. Part a·Krishanu SahaKevin E Healy
Sep 18, 2007·Journal of Neuroscience Research·Margarida SerraPaula M Alves
Jun 16, 2010·Cell Proliferation·B ShiM Guo
Feb 21, 2006·Biotechnology and Bioengineering·Jane A GilbertsonMichael S Kallos
Aug 16, 2011·Biotechnology and Applied Biochemistry·Carlos A V RodriguesJoaquim M S Cabral
Jul 1, 2017·Postȩpy higieny i medycyny doświadczalnej·Wiktoria Maria SuchorskaTomasz Trzeciak

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