PMID: 3942205Jan 1, 1986

Passive permeability of human red blood cells to calcium

The American Journal of Physiology
M K McNamara, J S Wiley

Abstract

Ca2+ influx was measured into human erythrocytes in which efflux was blocked by either introduction of an intracellular Ca2+ chelator, introduction of the Ca2+ chelator followed by ATP depletion, or depletion of the Ca2+ pump cofactors ATP and Mg2+. The Ca2+ influx under all three conditions was 14-20 mumol . 1 cells-1 . h-1, which is an order of magnitude higher than the influx previously reported for cells depleted of either ATP or Mg2+ separately. The difference between the two values was explained by the finding of substantial Ca2+ efflux from the Ca2+-loaded ATP-depleted cells, whereas this efflux was insignificant from cells loaded with quin 2 and then ATP depleted. Under these latter conditions Ca2+ influx estimates the unidirectional permeability to this cation. Studies using this technique showed that Ca2+ influx was the same in media of isotonic sodium, potassium, lithium, choline, or magnesium chlorides. Moreover the dependence of Ca2+ influx on external Ca2+ concentration was well described by the sum of saturable and nonsaturable (linear) components.

Citations

Feb 1, 1996·Pflügers Archiv : European journal of physiology·J S Huntley, A C Hall
Mar 29, 2001·Physiological Reviews·K Kirk
Jul 1, 1995·European Journal of Clinical Investigation·I EngströmA Waldenström
Jan 1, 1987·The Journal of Membrane Biology·B Engelmann, J Duhm
Jul 1, 1987·British Journal of Haematology·M K McNamara, J S Wiley
Mar 1, 1995·Critical Care Medicine·J C Todd, D L Mollitt
Feb 1, 1992·Hypertension·M David-DufilhoM A Devynck
Jan 1, 1988·Acta Medica Scandinavica·G Ronquist, G Frithz

Related Concepts

Quin2
Adenosine Triphosphate, Chromium Ammonium Salt
Aminoquinolines
Calcium
Erythrocytes
Magnesium
Mathematics
Permeability

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