Passive protection of gnotobiotic calves using monoclonal antibodies directed at different epitopes on the fusion protein of bovine respiratory syncytial virus

The Journal of Infectious Diseases
L H ThomasG Taylor

Abstract

Two neutralizing, fusion-inhibiting bovine monoclonal antibodies (MAbs; B4 and B13) directed at different epitopes on the fusion protein of respiratory syncytial virus (RSV) protected the lungs of gnotobiotic calves from RSV infection. The MAbs were administered intratracheally 24 h before the calves were challenged with bovine RSV. A third, nonneutralizing, non-fusion-inhibiting but complement-fixing MAb, B1, provided no significant protection against infection, and the disease was not exacerbated. Pneumonic consolidation and mean virus titer in lung 7 days after challenge were significantly lower in calves given the fusion-inhibiting MAbs than in either control calves or those given MAb B1. The proliferative bronchiolitis with syncytial formation and widespread distribution of RSV antigen in the lower respiratory tract of the B1-treated and control calves were indistinguishable and typical of experimental bovine RSV infection. Syncytia were markedly absent, and little or no viral antigen was detected in either the B4- or B13-treated calves.

Citations

Aug 1, 1995·Clinical and Experimental Immunology·A K Sharma, Z Woldehiwet
Jan 18, 2005·Vaccine·Geraldine TaylorLewis H Thomas
Jan 3, 2015·Molecular Immunology·Efrain Guzman, Geraldine Taylor
Dec 3, 2016·Vaccine·Geraldine Taylor
May 16, 2014·Clinical and Vaccine Immunology : CVI·Sara HägglundJean-François Eléouët
Jan 30, 2007·Veterinary Research·Jean-Francois Valarcher, Geraldine Taylor
Apr 6, 2007·Expert Review of Vaccines·Nicholas BennettJoseph Domachowske
Sep 29, 2009·Journal of Bacteriology·Alexandra NunesJoão P Gomes

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