Passive protection to bovine rotavirus (BRV) infection induced by a BRV VP8* produced in plants using a TMV-based vector

Archives of Virology
D M Pérez FilgueiraManuel V Borca

Abstract

We have previously reported on the use of a tobacco mosaic virus (TMV) vector TMV-30B to express foreign viral antigens for use as experimental immunogens. Here we describe the development of an improved TMV-30B vector that adds a sequence of 7 histidine residues to the C-terminus of recombinant proteins expressed in the vector. We used this TMV-30B-HISc vector to express the VP8* fragment of the VP4 protein from bovine rotavirus (BRV) strain C-486 in plants. Recombinant VP8* protein was purified from N. benthamiana leaves at 7 days post-inoculation by immobilized metal affinity chromatography. The plant-produced VP8* was initially detected using anti-His tag mAb and its antigenic nature was confirmed using both monoclonal and polyclonal specific antisera directed against BRV. Adult female mice, inoculated by the intraperinoteal route with an immunogen containing 4 microg of recombinant VP8*, developed a specific and sustained response to the native VP8* from the homologous BRV. Eighty five percent of suckling mice from immunized dams that were challenged with the homologous virus at the fifth day of age were protected from virus as compared to 35% of the pups from mothers immunized with a control protein. These results demonst...Continue Reading

Citations

Aug 21, 2010·Molecular Biotechnology·Louis-Philippe Bergeron-SandovalFathey Sarhan
Jan 8, 2013·Veterinary Immunology and Immunopathology·María Sol Peréz AguirreburualdeAndrés Wigdorovitz
Nov 1, 2008·Vaccine·Demian BellidoMaría José Dus Santos
Mar 18, 2010·Plant Biotechnology Journal·Edward P Rybicki
Nov 9, 2005·Protein Expression and Purification·Alexsandra R M FavachoVera S Gouvea
Sep 30, 2005·Biotechnology and Bioengineering·Isabel AndrésJesús Zueco
Feb 19, 2013·Expert Review of Vaccines·Nunzia Scotti, Edward P Rybicki
Jan 1, 2008·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·J J JoensuuJ E Brandle
Jun 21, 2006·Drugs in R&D·Vidadi YusibovRainer Fischer

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