PAT-Probabilistic Axon Tracking for Densely Labeled Neurons in Large 3-D Micrographs

IEEE Transactions on Medical Imaging
Henrik SkibbeShin Ishii

Abstract

A major goal of contemporary neuroscience research is to map the structural connectivity of mammalian brain using microscopy imaging data. In this context, the reconstruction of densely labeled axons from two-photon microscopy images is a challenging and important task. The visually overlapping, crossing, and often strongly distorted images of the axons allow many ambiguous interpretations to be made. We address the problem of tracking axons in densely labeled samples of neurons in large image data sets acquired from marmoset brains. Our high-resolution images were acquired using two-photon microscopy and they provided whole brain coverage, occupying terabytes of memory. Both the image distortions and the large data set size frequently make it impractical to apply present-day neuron tracing algorithms to such data due to the optimization of such algorithms to the precise tracing of either single or sparse sets of neurons. Thus, new tracking techniques are needed. We propose a probabilistic axon tracking algorithm (PAT). PAT tackles the tracking of axons in two steps: locally (L-PAT) and globally (G-PAT). L-PAT is a probabilistic tracking algorithm that can tackle distorted, cluttered images of densely labeled axons. L-PAT divid...Continue Reading

Citations

Mar 9, 2019·Frontiers in Neuroanatomy·Shiwei LiShaoqun Zeng
Aug 28, 2020·Frontiers in Neuroanatomy·Qing HuangTingwei Quan

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