PMID: 8972867Dec 1, 1996Paper

Pat1: a topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae

Nucleic Acids Research
X WangI D Hickson

Abstract

Saccharomyces cerevisiae top2 mutants deficient in topoisomerase II activity are defective in chromosome segregation during both mitotic and meiotic cell divisions. To identify proteins that act in concert with topoisomerase II during chromosome segregation in S.cerevisiae, we have used a two-hybrid cloning approach. We report the isolation of the PAT1 gene (for protein associated with topoisomerase II), which encodes a novel 90 kDa proline- and glutamine-rich protein that interacts with a highly conserved, leucine-rich region of topoisomerase II in vivo. Strains lacking Pat1p exhibit a slow growth rate and a phenotype reminiscent of conditional top2 mutants grown at the semi-permissive temperature; most notably, a reduced fidelity of chromosome segregation during both mitosis and meiosis. These findings indicate that the PAT1 gene is necessary for accurate chromosome transmission during cell division in eukaryotic cells and suggest that the interaction of Pat1p and topoisomerase II is an important component of this function.

References

Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J E Lindsley, J C Wang
Jan 1, 1985·Annual Review of Biochemistry·J C Wang
Jan 1, 1985·Annual Review of Cell Biology·A W Murray, J W Szostak
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S DiNardoR Sternglanz
Jul 27, 1995·Nature·G D'Arcangelo, T Curran
Oct 28, 1993·Nature·J C ChriviaR H Goodman

❮ Previous
Next ❯

Citations

Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Austin, K L Marsh
Sep 28, 1998·Biochimica Et Biophysica Acta·J L Nitiss
Nov 30, 2002·Biochimie·Hélène TourrièreJamal Tazi
Jan 3, 2003·Mutation Research·Felipe CortésInmaculada Domínguez
Apr 4, 2000·The EMBO Journal·E BouveretB Séraphin
May 29, 2002·The EMBO Journal·Nicole Fischer, Karsten Weis
Mar 7, 2003·Mutagenesis·Felipe Cortés, Nuria Pastor
Nov 9, 2001·Cell Structure and Function·R IshidaY Hiraoka
Dec 21, 2005·FEBS Letters·Nathalie A P Mouchel, John R Jenkins
Sep 14, 2012·Molecular Microbiology·Hee-Soo ParkJae-Hyuk Yu
Jan 15, 2010·Journal of Applied Microbiology·D StanleyG A Stanley
Feb 27, 2016·RNA Biology·Mridula MuppavarapuTracy Nissan
Feb 21, 2006·Biochemical and Biophysical Research Communications·Catherine R BarkerJohn R Jenkins
Aug 31, 2000·Annual Review of Biochemistry·M Tucker, R Parker
Apr 3, 2015·Molecular Biology of the Cell·Prashant K MishraMunira A Basrai
Oct 6, 1997·Archives of Biochemistry and Biophysics·D J Kroll
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·C J WiluszS W Peltz
Dec 1, 2001·Nature Genetics·C B BennettM A Resnick
Jun 25, 2019·Wiley Interdisciplinary Reviews. RNA·Caroline VindryNancy Standart
Sep 3, 1999·The Journal of Biological Chemistry·L BjergbaekA H Andersen
May 2, 2000·The Journal of Biological Chemistry·E U KurzF Gieseler
Feb 19, 2016·G3 : Genes - Genomes - Genetics·Mkhululi N MaphosaBrenda D Wingfield
Dec 2, 2010·Biochemical Society Transactions·Aline Marnef, Nancy Standart
Mar 13, 2001·Journal of Molecular Biology·D SikderV Nagaraja
Sep 28, 1998·Biochimica Et Biophysica Acta·R J IsaacsI D Hickson
Jul 19, 2014·RNA·Ashis ChowdhurySundaresan Tharun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.