PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides

Nature Chemical Biology
David SychanthaAnthony J Clarke

Abstract

O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1.

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Citations

Oct 9, 2019·International Journal of Molecular Sciences·Fiona F HagerChristina Schäffer
Jan 30, 2020·Nature Reviews. Microbiology·M Fata Moradali, Bernd H A Rehm
Jan 23, 2020·Scientific Reports·Rūta StanislauskienėRolandas Meškys
Oct 18, 2018·Frontiers in Microbiology·David SychanthaAnthony J Clarke
Sep 20, 2020·Nature Communications·Timm FiebigMartina Mühlenhoff
Sep 7, 2018·Frontiers in Plant Science·Markus Pauly, Vicente Ramírez
Oct 28, 2020·Journal of Applied Microbiology·V S ShadrinG V Mikoulinskaia
Dec 2, 2020·Microorganisms·Alice ChateauAntonella Fioravanti
Mar 12, 2020·ACS Infectious Diseases·Laura DabosThierry Naas

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