Patch-clamp amplifiers on a chip.

Journal of Neuroscience Methods
P WeerakoonF J Sigworth

Abstract

We present the first, fully integrated, two-channel implementation of a patch-clamp measurement system. With this "PatchChip" two simultaneous whole-cell recordings can be obtained with rms noise of 8pA in a 10kHz bandwidth. The capacitance and series-resistance of the electrode can be compensated up to 10pF and 100MΩ respectively under computer control. Recordings of hERG and Na(v) 1.7 currents demonstrate the system's capabilities, which are on par with large, commercial patch-clamp instrumentation. By reducing patch-clamp amplifiers to a millimeter size micro-chip, this work paves the way to the realization of massively parallel, high-throughput patch-clamp systems for drug screening and ion-channel research. The PatchChip is implemented in a 0.5μm silicon-on-sapphire process; its size is 3×3mm(2) and the power consumption is 5mW per channel with a 3.3V power supply.

References

Feb 1, 1995·Journal of Neuroscience Methods·F J SIGWorth
Dec 1, 1995·The Journal of General Physiology·F J Sigworth
Apr 1, 2009·IEEE Transactions on Biomedical Circuits and Systems·P WeerakoonF J Sigworth

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Citations

Dec 14, 2011·Sensors·Benoit Gosselin
Jul 16, 2013·IEEE Transactions on Biomedical Circuits and Systems·Jungsuk KimWilliam B Dunbar
Jul 16, 2013·IEEE Transactions on Biomedical Circuits and Systems· Dongsoo KimE Culurciello
Jan 23, 2015·Frontiers in Genetics·Yue WangZhimin Wang
May 23, 2014·Lab on a Chip·Brandon R BruhnMichael Mayer
Nov 28, 2014·Journal of Neurophysiology·Reid R HarrisonCraig Forest
May 26, 2020·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Chon Lok LeiGary R Mirams
Dec 19, 2019·Nature Electronics·Siddharth ShekarKenneth L Shepard

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