Patch clamp recording from the intact dorsal root ganglion

Journal of Neuroscience Methods
J M ZhangRobert H Lamotte

Abstract

A method for patch-clamp recording from intact dorsal root ganglion (DRG) cells in rat is described. The L4 and L5 DRGs with sciatic nerve attached were excised from rats (10-15 days old) and placed in a recording chamber after removing the ganglion sheath and dissolving the connective tissue with dilute collagenase. The somata of individual cells were exposed by gentle surface cleaning through a perfusion micropipette. Somata were classified as Abeta, Adelta or C based on the cell size and the shape of the action potential (AP). Under current clamp, axonal conduction velocity (CV) was calculated from the distance between a stimulating electrode and the center of the ganglion divided by the latency of the AP elicited by stimulation of the sciatic nerve. CVs ranged from 0.2-0.8 m/s for C cells, 0.8-2.4 for Adelta and 3.2-5.0 for A/beta cells. AP threshold occurred at a significantly more positive potential in C cells than in Adelta and Abeta cells. Under voltage clamp, sodium currents were recorded from C cells. Both TTX-resistant (TTX-R) and TTX-sensitive (TTX-S currents) were demonstrated in the present study. The results demonstrate the feasibility of patch-clamp recording from intact, identified DRG cells in vitro.

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Citations

May 3, 2005·Journal of Pharmacological and Toxicological Methods·Gayle M Passmore
May 7, 2002·Journal of Neuroscience Methods·B Y Li, J H Schild
Feb 24, 2001·The European Journal of Neuroscience·F VianaC Belmonte
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Aug 2, 2013·International Journal of Biological Sciences·Xiao-Long LuBai-Yan Li
Aug 22, 2006·European Journal of Pain : EJP·Forrest L Smith, Raymond J Lindsay
Nov 25, 2006·Seminars in Cell & Developmental Biology·Marc RogersEdward B Stevens
May 26, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·E CiglieriC Salio
Apr 5, 2017·The Journal of Clinical Investigation·Xiaona DuNikita Gamper
Sep 25, 2018·Neuromodulation : Journal of the International Neuromodulation Society·Timothy R DeerNagy Mekhail
Jun 17, 2006·Journal of Applied Physiology·Edward Vincent S Faustino, David F Donnelly
Sep 4, 2015·Journal of Neurophysiology·Danielle SundtDavid B Jaffe
Jan 27, 2019·Brain Sciences·Timothy R DeerKrishnan Chakravarthy
Apr 15, 2019·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Robert D GrahamScott F Lempka

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