PMID: 8606363Nov 1, 1995Paper

Patch clamp study of the voltage-dependent anion channel in the thylakoid membrane

The Journal of Membrane Biology
I I Pottosin, G Schönknecht

Abstract

Measurements of single channel currents were performed on isolated membrane patches from osmotically swollen thylakoids of the Charophyte alga Nitellopsis obtusa. A channel with a high selectivity for anions over cations and a conductance of 100 to 110 pS (114 mm Cl-) was revealed. The channel has a bell-shaped voltage-dependence of the open probability, with a maximum at about 0 mV. This dependence was explained by two gating processes, one causing channel closure at positive and one at negative potentials. The steepness of the voltage-dependence corresponded to approximately 2 elementary charges to be transferred across the entire membrane in each of the two gating processes. The analysis of the anion channel kinetics in the millisecond time domain revealed an e-fold increase of mean open and decrease of mean closed times when the membrane voltage was made more positive by 20 and 36 mV, respectively. Concert transitions of two identical anion channels between open and long inactivated states were observed, while the millisecond closed-open transitions of the two channels within a burst of activity were kinetically independent.

Citations

Jul 13, 2006·Planta·Rainer Hedrich, Irene Marten
Sep 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·John M A GrimePatrick R Unwin
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·C MaurelP Ripoche
Jan 2, 2008·The Journal of General Physiology·John C MathaiMark L Zeidel
Jan 2, 2008·The Journal of General Physiology·John F NagleStephanie Tristram-Nagle
Jun 9, 2000·Annual Review of Physiology·A J Hulbert, P L Else
Jul 10, 2013·Cellular and Molecular Life Sciences : CMLS·Bernard E PfeilCornelia Spetea
Jan 7, 2016·Frontiers in Physiology·Igor Pottosin, Oxana Dobrovinskaya
Jul 31, 2013·Biophysical Chemistry·Vanessa ChecchettoIldiko Szabo
Mar 8, 2016·Journal of Integrative Plant Biology·Zhikun DuanLianwei Peng
Jul 20, 2017·The New Phytologist·Rainer Hedrich, Dietmar Geiger
Mar 15, 2013·International Journal of Molecular Sciences·Hyun Ji ParkHye Sun Cho
May 25, 2016·Nature Communications·Andrei HerdeanBjörn Lundin
Oct 18, 2012·Physiological Reviews·Rainer Hedrich
Nov 17, 2010·The Journal of General Physiology·Horia VaisDon-On Daniel Mak
Apr 18, 1998·The Journal of Biological Chemistry·T KieselbachW P Schröder
Nov 24, 2001·The Journal of Biological Chemistry·Maria SchubertThomas Kieselbach
Feb 23, 2017·Frontiers in Pharmacology·Christian GrimmMartin Biel
Oct 28, 2017·Scientific Reports·George ShapovalovNatalia Prevarskaya
Mar 16, 2018·Cellular and Molecular Life Sciences : CMLS·Justine MarchandCornelia Spetea
Nov 29, 2005·Journal of Chemical Information and Modeling·Anna SegallaGiorgio Mario Giacometti

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