PMID: 7544139Aug 1, 1995Paper

Patch sensor detection of glutamate release evoked by a single electrical shock

Neuron
T MaedaM Satoh

Abstract

We tried to detect minimal stimulation-induced glutamate overflow from the surface of a hippocampal slice using an outside-out patch electrode excised from pyramidal cell membranes. The amplitude of the stimulation-induced patch current was dependent on the distance between the slice surface and the tip of patch sensor. The current-voltage relations of the stimulation-induced patch current were similar to those of the current evoked puff by application of L-glutamate to the patch. This indicates that the stimulation-induced patch current was produced by glutamate released from presynaptic terminals, and thus this technique may be useful in the study of transmitter release evoked by minimal electrical stimulation in brain slices.

References

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Citations

Nov 13, 2004·Science's STKE : Signal Transduction Knowledge Environment·Seiji HayashiYasunobu Okada
Feb 1, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kumiko NakajimaMasao Sugawara
Mar 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takayuki OkaMasao Sugawara
Dec 1, 2007·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Masao Sugawara
Feb 13, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Natsumi SomaMasao Sugawara
May 5, 2009·Neuron·Aaron G BlankenshipMarla B Feller
Jun 18, 2010·Journal of Neurophysiology·Miriam Ivenshitz, Menahem Segal
Jan 15, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Masao SugawaraMisato Sakamoto

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