PMID: 1214276Dec 4, 1975Paper

Patch voltage clamp of squid axon membrane

The Journal of Membrane Biology
H M Fishman

Abstract

A small area (patch) of the external surface of a squid axon can be "isolated" electrically from the surrounding bath by means of a pair of concentric glass pipettes. The seawater-filled inner pipette makes contact with the axon and constitutes the external access to the patch. The outer pipette is used to direct flowing sucrose solution over the area surrounding the patch of membrane underlying the inner pipette. Typically, sucrose isolated patches remain in good condition (spike amplitude greater than 90 mV) for periods of approximately one half hour. Patches of axon membrane which had previously been exposed to sucrose solution were often excitable. Membrane survival of sucrose treatment apparently arises from an outflow of ions from the axon and perhaps satellite cells into the interstitial cell space surrounding the exolemma. Estimate of the total access resistance (electrode plus series resistance) to the patch is about 100 komega (7 omega cm2). Patch capacitance ranges from 10-100 pF, which suggests areas of 10(-4) to 10(-5) cm2 and resting patch resistances of 10-100 Momega. Shunt resistance through the interstitial space exposed to sucrose solution, which isolates the patch, is typically 1-2 Momega. These parameters in...Continue Reading

References

Dec 4, 1975·The Journal of Membrane Biology·H M FishmanL E Moore
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H M Fishman
Sep 1, 1973·IEEE Transactions on Bio-medical Engineering·H M Fishman
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Citations

Oct 1, 1984·Pflügers Archiv : European journal of physiology·W M Roberts, W Almers
Jul 18, 1978·Pflügers Archiv : European journal of physiology·E NeherJ H Steinbach
Dec 4, 1975·The Journal of Membrane Biology·H M FishmanL E Moore
Dec 4, 1975·The Journal of Membrane Biology·H M FishmanD M Poussart
May 21, 1979·The Journal of Membrane Biology·L E MooreD J Poussart
Jan 1, 1985·Progress in Biophysics and Molecular Biology·H M Fishman
Feb 1, 1980·Journal of Neuroscience Methods·K S LeeA M Brown
Dec 1, 1988·Journal of Neuroscience Methods·I C Forster, N G Greeff
Jun 1, 1986·The Journal of General Physiology·J H CaldwellK G Beam
May 1, 1990·The Journal of General Physiology·P Shrager, C T Rubinstein
Apr 28, 1978·Annals of the New York Academy of Sciences·A M BrownK S Lee
Feb 24, 1977·Nature·K S LeeA M Brown

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