Paternal mitochondrial DNA transmission during nonhuman primate nuclear transfer

Genetics
Justin C St John, Gerald Schatten

Abstract

Offspring produced by nuclear transfer (NT) have identical nuclear DNA (nDNA). However, mitochondrial DNA (mtDNA) inheritance could vary considerably. In sheep, homoplasmy is maintained since mtDNA is transmitted from the oocyte (recipient) only. In contrast, cattle are heteroplasmic, harboring a predominance of recipient mtDNA along with varying levels of donor mtDNA. We show that the two nonhuman primate Macaca mulatta offspring born by NT have mtDNA from three sources: (1) maternal mtDNA from the recipient egg, (2) maternal mtDNA from the egg contributing to the donor blastomere, and (3) paternal mtDNA from the sperm that fertilized the egg from which the donor blastomere was isolated. The introduction of foreign mtDNA into reconstructed recipient eggs has also been demonstrated in mice through pronuclear injection and in humans through cytoplasmic transfer. The mitochondrial triplasmy following M. mulatta NT reported here forces concerns regarding the parental origins of mtDNA in clinically reconstructed eggs. In addition, mtDNA heteroplasmy might result in the embryonic stem cell lines generated for experimental and therapeutic purposes ("therapeutic cloning").

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Citations

Mar 3, 2005·Reproduction, Fertility, and Development·Carol A BrennerKenneth E Pierce
Nov 2, 2006·DNA Sequence : the Journal of DNA Sequencing and Mapping·Gen Hua YueFelicia Feng
Sep 6, 2005·Molecular Reproduction and Development·Justin C St JohnNatasha D'Souza
Mar 25, 2006·Stem Cells·Vanessa J HallMiodrag Stojkovic
Jul 21, 2007·Stem Cells·Christopher S NavaraGerald Schatten
Sep 5, 2006·Lancet·J PoultonJ St John
Nov 5, 2013·Biochimica Et Biophysica Acta·Justin St John
Nov 27, 2014·Molecular Human Reproduction·Joerg Patrick BurgstallerJoanna Poulton
Dec 4, 2009·Biology of Reproduction·Christina Ramires FerreiraRalf Steinborn
Aug 12, 2010·Cellular Reprogramming·Joon-Hee LeeKeith H S Campbell
Mar 19, 2020·Journal of Internal Medicine·W Wei, P F Chinnery
Apr 4, 2006·Human Reproduction Update·E C SpikingsJ C St John
Apr 10, 2020·Nature Communications·Wei WeiPatrick F Chinnery
Dec 9, 2020·Human Reproduction·Hong MaShoukhrat Mitalipov
May 21, 2005·Science·Woo Suk HwangGerald Schatten

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