PMID: 11331620May 2, 2001Paper

Paternal monoallelic expression of PEG3 in the human placenta

Human Molecular Genetics
S E HibyA King

Abstract

Genomic imprinting is the phenomenon whereby mono-allelic expression of certain genes occurs depending on their parental origin. The observation that imprinting only occurs in placental mammals has led to the suggestion that it may play a role in this form of reproduction. In the present study we have investigated the pattern of expression of the human PEG3 gene in the early to term placenta, as well as the uterus and ovary, using RT-PCR, northern blot and in situ hybridization. A comparison is made with the expression of Peg3 in the mouse by histochemical staining in betageo knock out mice. We have demonstrated high levels of PEG3 in the human placenta and have localized the signal to the layer of villous cytotrophoblast cells. In contrast, the pattern of expression of Peg3 in the mouse placenta is less restricted, the message being present in all trophoblast populations. Thus, expression of PEG3/Peg3 in the human and mouse placenta is not directly comparable. We have also detected PEG3 message in the ovarian stroma. We have sequenced the human PEG3 gene from exon 3 to exon 9. By utilizing a polymorphism detected in exon 9, we have established that only the paternal allele is expressed in human placenta. Human PEG3 is therefor...Continue Reading

Citations

Jul 19, 2002·Journal of Cellular Physiology·Benjamin Tycko, Ian M Morison
Jan 5, 2006·Immunogenetics·Anita E TrundleyAshley Moffett
Apr 20, 2005·Placenta·P M CoanA C Ferguson-Smith
Jul 9, 2010·Human Molecular Genetics·Marie Noguer-DanceJérôme Cavaillé
Jun 25, 2004·Biology of Reproduction·Tom P FlemingJudith J Eckert
May 24, 2005·Journal of Medical Genetics·K A Kaiser-RogersW P Robinson
Oct 22, 2010·The Journal of Clinical Endocrinology and Metabolism·Liis UuskülaMaris Laan
Jun 7, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Neelam A Kedia-MokashiN H Balasinor
Nov 6, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Christelle Stouder, Ariane Paoloni-Giacobino
Mar 1, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·S J TunsterR M John
May 9, 2012·Reproductive Biomedicine Online·Ionel SandoviciMiguel Constância
Nov 27, 2010·Progress in Biophysics and Molecular Biology·A L FowdenM Constancia
Jan 15, 2011·Animal Reproduction Science·Q Y ZhouS H Zhao
Apr 29, 2008·Frontiers in Neuroendocrinology·Eric B Keverne, James P Curley
Dec 4, 2003·Tissue Antigens·A Trundley, A Moffett
Jul 29, 2010·Prenatal Diagnosis·Kiyonori MiuraHideaki Masuzaki
May 19, 2007·Genomics·Joomyeong KimLisa Stubbs
Mar 9, 2018·The Journal of Experimental Biology·Féaron C Cassidy, Marika Charalambous
Apr 1, 2006·Cytogenetic and Genome Research·B Tycko
Oct 31, 2006·The Journal of Biological Chemistry·Ying-Chun LuRobert Chiu
Jun 2, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J P CurleyE B Keverne
Jun 6, 2020·Journal of Neuroendocrinology·Peter A CattiniMargaret E Bock
Jan 21, 2009·Reproduction, Fertility, and Development·A Dinnyes, T L Nedambale
Jul 7, 2017·Biology of Reproduction·Hongen XuKrzysztof Flisikowski
Oct 16, 2018·Frontiers in Cell and Developmental Biology·Simon J TunsterRosalind M John
Mar 17, 2006·Human Reproduction Update·I B Van den Veyver, T K Al-Hussaini
Oct 18, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nilesh L GardiSharmila A Bapat
Jan 14, 2020·Molecular Therapy. Nucleic Acids·Anna Kotowska-ZimmerMarta Olejniczak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.