Jul 29, 2010

Paternally biased X inactivation in mouse neonatal brain

Genome Biology
Xu WangAndrew G Clark

Abstract

X inactivation in female eutherian mammals has long been considered to occur at random in embryonic and postnatal tissues. Methods for scoring allele-specific differential expression with a high degree of accuracy have recently motivated a quantitative reassessment of the randomness of X inactivation. After RNA-seq data revealed what appeared to be a chromosome-wide bias toward under-expression of paternal alleles in mouse tissue, we applied pyrosequencing to mouse brain cDNA samples from reciprocal cross F1 progeny of divergent strains and found a small but consistent and highly statistically significant excess tendency to under-express the paternal X chromosome. The bias toward paternal X inactivation is reminiscent of marsupials (and extraembryonic tissues in eutherians), suggesting that there may be retained an evolutionarily conserved epigenetic mark driving the bias. Allelic bias in expression is also influenced by the sampling effect of X inactivation and by cis-acting regulatory variation (eQTL), and for each gene we quantify the contributions of these effects in two different mouse strain combinations while controlling for variability in Xce alleles. In addition, we propose an efficient method to identify and confirm g...Continue Reading

  • References47
  • Citations21

References

Mentioned in this Paper

Embryo
Neuro-Oncological Ventral Antigen 2
Quantitative Trait Loci
KDM5C gene
CAB39L gene
CSTF2 gene
MED14 gene
C57BL/6 Mouse
Genus Cis
WDR13 gene

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