Paternity assessment in rhesus macaques (Macaca mulatta): multilocus DNA fingerprinting and PCR marker typing

American Journal of Primatology
P NürnbergM Krawczak

Abstract

Establishing kinship relations in primates using modern molecular genetic techniques has enhanced the ability to scrutinize a number of fundamental biological issues. We screened 51 human short tandem repeats (STRs) for cross-species PCR amplification in rhesus macaques (Macaca mulatta) and identified 11 polymorphic loci with heterozygosity rates of at least 0.6. These markers were used for paternity testing in three social groups (M, R, and S) of rhesus macaques from Cayo Santiago, Puerto Rico. Several consecutive birth cohorts were analyzed in which approximately 200 males were tested for paternity against more than 100 mother/ infant pairs. Despite a combined exclusion rate of more than 99.9% in all three groups, some cases could not be solved unequivocally with the STR markers and additional testing of the MHC-associated DQB1 polymorphism. A final decision became possible through multilocus DNA fingerprinting with one or more of the oligonucleotide probes (GATA)4, (CA)8, and (CAC)5. Paternity assessment by multilocus DNA analysis with probe (CAC)5 alone was found to have limitations in rhesus macaques as regards the number of potential sires which might be involved in a given case. Multilocus DNA fingerprinting requires lar...Continue Reading

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Citations

Jul 17, 2004·Proceedings. Biological Sciences·Anja WiddigMichael Krawczak
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·A WiddigF B Bercovitch
Jul 16, 2014·Behavioral Ecology : Official Journal of the International Society for Behavioral Ecology·Constance DubucAnja Widdig
Jun 3, 2015·American Journal of Primatology·Anja WiddigJörg Schmidtke
Apr 27, 2002·American Journal of Primatology·Fred B BercovitchMichael Krawczak
May 20, 2015·International Journal of Primatology·Lars KulikAnja Widdig
Mar 11, 2000·American Journal of Primatology·I ClissonB Crouau-Roy
Jun 21, 2006·American Journal of Primatology·Matthew J KesslerJanis Gonzalez-Martinez
Feb 23, 2008·Behavior Genetics·Marie J E CharpentierAnja Widdig
Sep 19, 2008·Human Mutation·Andreas WolfDavid N Cooper
Aug 30, 2011·Behavioral Ecology and Sociobiology·Constance DubucAnja Widdig

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