Pathogenesis of corneal infection: binding of Pseudomonas aeruginosa to specific phospholipids.

Infection and Immunity
N PanjwaniF Jungalwala

Abstract

Clinical isolates of Pseudomonas aeruginosa were examined for binding interactions with phospholipids of corneal epithelium. Thin-layer chromatography (TLC) of lipids extracted from corneal epithelia followed by staining with an ammonium molybdate spray reagent revealed three phospholipid components, PL1, PL2, and PL3. The chromatographic mobility of PL1 was similar to that of the phospholipid standards phosphatidylinositol (PI) and phosphatidylserine (PS), which were not well resolved from one other; PL2 and PL3 comigrated with the standards phosphatidylcholine and phosphatidylethanolamine, respectively. By use of a TLC-bacterial overlay procedure, 35S-labeled P. aeruginosa organisms were shown to bind to PL1 but not to PL2 or PL3. P. aeruginosa binding to PL1 was concentration dependent. Alkaline methanolysis abolished the binding. PL1 was separated into two components, PL1-I and PL1-S, by chromatography on borate-treated TLC plates. Both PL1-I and PL1-S contained binding sites for P. aeruginosa. Mass spectral analysis identified PL1-I and PL1-S as PI and PS, respectively. Radiolabeled P. aeruginosa organisms were subsequently shown to bind to commercially available bovine PI and PS and synthetic dipalmitoyl-PS but not to oth...Continue Reading

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Citations

May 8, 2004·Letters in Applied Microbiology·R A BojarK T Holland
Jan 30, 2008·Microbiology·Jayasimha RaoJoanna B Goldberg
Nov 2, 2011·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Negar Babaei OmaliMark D P Willcox
Mar 13, 2002·Paediatric Drugs·Serina StrettonMark D P Willcox
Jan 31, 2008·Analytical Chemistry·Gerald StübigerGünter Allmaier

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