Pathogenesis of Shigella diarrhea: rabbit intestinal cell microvillus membrane binding site for Shigella toxin.

Infection and Immunity
G FuchsG T Keusch

Abstract

This study examined the binding of purified 125I-labeled shigella toxin to rabbit jejunal microvillus membranes (MVMs). Toxin binding was concentration dependent, saturable, reversible, and specifically inhibited by unlabeled toxin. The calculated number of toxin molecules bound at 4 degrees C was 7.9 X 10(10) (3 X 10(10) to 2 X 10(11))/micrograms of MVM protein or 1.2 X 10(6) per enterocyte. Scatchard analysis showed the binding site to be of a single class with an equilibrium association constant, K, of 4.7 X 10(9) M-1 at 4 degrees C. Binding was inversely related to the temperature of incubation. A total of 80% of the labeled toxin binding at 4 degrees C dissociated from MVM when the temperature was raised to 37 degrees C, but reassociated when the temperature was again brought to 4 degrees C. There was no structural or functional change of MVM due to toxin as monitored by electron microscopy or assay of MVM sucrase activity. These studies demonstrate a specific binding site for shigella toxin on rabbit MVMs. The physiological relevance of this receptor remains to be determined.

References

Jan 1, 1985·Ciba Foundation Symposium·G T KeuschM Jacewicz
Sep 1, 1985·Pediatric Research·M M JonasR J Grand
May 1, 1972·The Journal of Infectious Diseases·G T KeuschS Z Hirschman
Mar 1, 1973·The Journal of Infectious Diseases·M M LevineJ P Libonati
May 1, 1969·Experimental Cell Research·D D Harrison, H L Webster
Mar 1, 1971·Biochimica Et Biophysica Acta·R J GrandK J Isselbacher
Jan 1, 1980·Journal of Receptor Research·K Eiklid, S Olsnes

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Citations

May 6, 2015·Glycobiology·Amr El-HawietJohn S Klassen
Aug 8, 2002·Hybridoma and Hybridomics·Tsuyoshi KimuraTae Takeda
Dec 22, 2009·Nature Reviews. Microbiology·Ludger Johannes, Winfried Römer
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dingwei DaiW Allan Walker
Nov 28, 2001·The Journal of Biological Chemistry·Anna M SoltykJames L Brunton
May 30, 2001·Microbes and Infection·E V O'Loughlin, R M Robins-Browne
Jan 1, 1989·Clinical Microbiology Reviews·M A Karmali
Jun 1, 1992·Veterinary Microbiology·H ImberechtsP Lintermans
May 1, 1996·Infection and Immunity·T E WaddellC L Gyles
Jun 1, 1987·Microbiological Reviews·A D O'Brien, R K Holmes
Sep 13, 2005·Biochimica Et Biophysica Acta·Wayne I Lencer, David Saslowsky
Aug 2, 2006·Veterinary Immunology and Immunopathology·Etienne MoussayChristian Menge

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