Pathogenic diversity among Chlamydia trachomatis ocular strains in nonhuman primates is affected by subtle genomic variations

The Journal of Infectious Diseases
Laszlo KariHarlan D Caldwell

Abstract

Chlamydia trachomatis is the etiological agent of trachoma, the leading cause of preventable blindness. Trachoma presents distinct clinical syndromes ranging from mild and self-limiting to severe inflammatory disease. The underlying host and pathogen factors responsible for these diverse clinical outcomes are unclear. To assess the role played by pathogen variation in disease outcome, we analyzed the genomes of 4 trachoma strains representative of the 3 major trachoma serotypes, using microarray-based comparative genome sequencing. Outside of ompA, trachoma strains differed primarily in a very small subset of genes (n = 22). These subtle genetic variations were manifested in profound differences in virulence as measured by in vitro growth rate, burst size, plaque morphology, and interferon-gamma sensitivity but most importantly in virulence as shown by ocular infection of nonhuman primates. Our findings are the first to identify genes that correlate with differences in pathogenicity among trachoma strains.

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Citations

Nov 23, 2011·Nature Reviews. Rheumatology·Eric Gracey, Robert D Inman
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Laszlo KariHarlan D Caldwell
Feb 16, 2011·The Journal of Infectious Diseases·Sandra G MorrisonRichard P Morrison
Jul 28, 2011·The Journal of Infectious Diseases·Isao MiyairiGerald I Byrne
Jan 7, 2009·Sexually Transmitted Diseases·João Paulo GomesMaria José Borrego
Jan 13, 2010·Antimicrobial Agents and Chemotherapy·Rachel BinetRoger G Rank
May 28, 2009·Infection and Immunity·Kyle H RamseyRoger G Rank
Mar 31, 2010·Infection and Immunity·Lacey D TaylorHarlan D Caldwell
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Sep 11, 2012·Journal of Bacteriology·Vítor BorgesJoão P Gomes
Nov 8, 2013·Journal of Clinical Microbiology·Anna R LastMartin J Holland
Dec 20, 2008·BMC Infectious Diseases·Erika LyytikäinenJorma Paavonen
Oct 25, 2008·Genome Biology·Alexandra NunesJoão P Gomes
Sep 30, 2008·PLoS Neglected Tropical Diseases·Aura A AndreasenRobin L Bailey
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Jan 23, 2009·FEMS Immunology and Medical Microbiology·Robert C Brunham, Michael L Rekart

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