Pathogenic E. coli exploits SslE mucinase activity to translocate through the mucosal barrier and get access to host cells

PloS One
Maria ValeriMarco Soriani

Abstract

SslE is a zinc-metalloprotease involved in the degradation of mucin substrates and recently proposed as a potential vaccine candidate against pathogenic E. coli. In this paper, by exploiting a human in vitro model of mucus-secreting cells, we demonstrated that bacteria expressing SslE have a metabolic benefit which results in an increased growth rate postulating the importance of this antigen in enhancing E. coli fitness. We also observed that SslE expression facilitates E. coli penetration of the mucus favouring bacteria adhesion to host cells. Moreover, we found that SslE-mediated opening of the mucosae contributed to the activation of pro-inflammatory events. Indeed, intestinal cells infected with SslE-secreting bacteria showed an increased production of IL-8 contributing to neutrophil recruitment. The results presented in this paper conclusively designate SslE as an important colonization factor favouring E. coli access to both metabolic substrates and target cells.

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Citations

Nov 3, 2015·Environmental Microbiology·Lucia Gonzales-Siles, Åsa Sjöling
Sep 14, 2016·Frontiers in Microbiology·Mani H VemulaSharmistha Banerjee
Jun 3, 2015·Infection and Immunity·Sabrina I GreenAnthony W Maresso
Jul 7, 2020·FEMS Microbiology Reviews·Ashley Valle Arevalo, Clarissa J Nobile
Jan 13, 2018·FEMS Microbiology Reviews·Pascale VonaeschPhilippe J Sansonetti
Jul 14, 2018·NPJ Biofilms and Microbiomes·Cassie R BakshaniJ Grant Burgess
Jun 5, 2019·FEMS Microbiology Reviews·Lucie Etienne-MesminStéphanie Blanquet-Diot
Jul 1, 2020·Scientific Reports·Lixia LiuFernando Ruiz-Perez
Feb 10, 2021·Cancers·Olivia I Coleman, Dirk Haller
Apr 29, 2021·The Biochemical Journal·D Judy ShonStacy A Malaker
Sep 30, 2021·Proceedings of the National Academy of Sciences of the United States of America·Daniel J HonigfortKamil Godula

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Methods Mentioned

BETA
PCR
density gradient centrifugation
flow cytometry
confocal microscopy

Software Mentioned

GraphPad Prism
Floujo

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