Pathogenic Escherichia coli and lipopolysaccharide enhance the expression of IL-8, CXCL5, and CXCL10 in canine endometrial stromal cells

Theriogenology
Iulia KarlssonSara Wernersson

Abstract

Chemokines play a central role in cellular communication in response to bacterial infection. However, the knowledge of the chemokine responses to bacterial infections in dogs remains limited. Uterine bacterial infection (pyometra) is one of the most common bacterial diseases in dogs and causes sepsis in most of the cases. We have shown previously that dogs with pyometra have higher messenger RNA (mRNA) levels of chemokines in uterus. To assess whether the stromal part of the endometrium expresses chemokines in response to bacterial infection, we cultured endometrial stromal cells isolated from healthy dogs and exposed them to either live pathogenic Escherichia coli, isolated from the uterus of a dog with pyometra, or lipopolysaccharide. Changes in the mRNA expression of ELR(+) CXC chemokines, IL-8, CXCL5, CXCL7, and ELR(-) CXC chemokine, CXCL10, were measured after 24 hours using quantitative real-time polymerase chain reaction. Levels of IL-8, CXCL5, and CXCL10 were upregulated in endometrial stromal cells exposed to E coli and lipopolysaccharide, whereas the level of CXCL7 was decreased or unaffected. In addition, levels of IL-8 and CXCL5, but not CXCL7 or CXCL10, were significantly higher in dogs with pyometra than those in ...Continue Reading

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Citations

Feb 4, 2016·Veterinary Immunology and Immunopathology·Iulia KarlssonSara Wernersson
Nov 11, 2016·Veterinary Research·Sofia HenriquesLuisa Mateus
Sep 7, 2019·Journal of Zhejiang University. Science. B·Hua ZhangDong-Mei Shi
Aug 18, 2017·Reproduction in Domestic Animals = Zuchthygiene·R Hagman
Jun 18, 2020·Frontiers in Veterinary Science·Roberta TroiaRobert Goggs

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