Pathogenic potential of Escherichia coli clinical strains from orthopedic implant infections towards human osteoblastic cells

Pathogens and Disease
Lise CrémetNathalie Caroff

Abstract

Escherichia coli is one of the first causes of Gram-negative orthopedic implant infections (OII), but little is known about the pathogenicity of this species in such infections that are increasing due to the ageing of the population. We report how this pathogen interacts with human osteoblastic MG-63 cells in vitro, by comparing 20 OII E. coli strains to two Staphylococcus aureus and two Pseudomonas aeruginosa strains. LDH release assay revealed that 6/20 (30%) OII E. coli induced MG-63 cell lysis whereas none of the four control strains was cytotoxic after 4 h of coculture. This high cytotoxicity was associated with hemolytic properties and linked to hlyA gene expression. We further showed by gentamicin protection assay and confocal microscopy that the non-cytotoxic E. coli were not able to invade MG-63 cells unlike S. aureus strains (internalization rate <0.01% for the non-cytotoxic E. coli versus 8.88 ± 2.31% and 4.60 ± 0.42% for both S. aureus). The non-cytotoxic E. coli also demonstrated low adherence rates (<7%), the most adherent E. coli eliciting higher IL-6 and TNF-α mRNA expression in the osteoblastic cells. Either highly cytotoxic or slightly invasive OII E. coli do not show the same infection strategies as S. aureus...Continue Reading

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Citations

Apr 4, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L CrémetN Caroff
Jan 11, 2020·Frontiers in Cellular and Infection Microbiology·Agata LosPaula Bourke
Aug 18, 2020·Virulence·Hala O EltwisyMohamed A El-Mokhtar
May 1, 2021·International Journal of Molecular Sciences·Ingmar A J van HengelAmir A Zadpoor
Jul 6, 2021·Saudi Journal of Biological Sciences·Zuzana AndrejčákováAlexander V Sirotkin
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marta LimaFilipe J Mergulhão
Jan 16, 2018·ACS Biomaterials Science & Engineering·Yeongseon JangJulie A Champion

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