Pathogenicity-associated islands in extraintestinal pathogenic Escherichia coli are fitness elements involved in intestinal colonization.

Journal of Bacteriology
Médéric DiardIvan Matic

Abstract

The virulence of many human pathogens does not seem to be an evolutionarily selected trait, but an accidental by-product of the selection that operates in another ecological context. We investigated the possibility that virulence of the extraintestinal pathogenic Escherichia coli (ExPEC) strains, which frequently cause disease in the host in which they asymptomatically colonize the intestine, is the consequence of commensalism. Most of the ExPEC virulence factors are clustered on genomic islands called pathogenicity-associated islands (PAIs). We constructed and characterized several mutants of the ExPEC 536 strain with either (i) deletions of each single PAI or (ii) a complete deletion of all seven PAIs. In vitro phenotypic characterization of 536 mutants showed that the seven PAIs were dispensable for growth in the absence of external stress, as well as under a range of biologically relevant stressors, i.e., serum, bile, and oxidative, nitrosative, hyperosmotic, and acidic stress. However, challenge against the wild-type (WT) strain in a murine model shows that the deletion of all seven PAIs drastically reduces the fitness of 536 during persistent intestinal colonization. This defect seems to be linked to the hypermotility obs...Continue Reading

References

Jan 1, 1977·Annual Review of Microbiology·D C Savage
May 1, 1975·Acta paediatrica Scandinavica·U LindbergU Jodal
Feb 1, 1983·Infection and Immunity·R FreterK E Carey
Dec 1, 1995·Infection and Immunity·M V HeríasA E Wold
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
Apr 1, 1996·Emerging Infectious Diseases·B R Levin
Jan 15, 2000·Infection and Immunity·J R Johnson, M Kuskowski
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Apr 11, 2001·Epidemiology and Infection·F NowrouzianA E Wold
Apr 11, 2002·The Journal of Laboratory and Clinical Medicine·James R Johnson, Thomas A Russo
Jun 1, 2002·Applied and Environmental Microbiology·D DjordjevicL A McLandsborough
Nov 1, 2002·Journal of Clinical Microbiology·Lixin ZhangCarl Marrs
Jul 5, 2003·Science·Gregory G AndersonScott J Hultgren
Dec 15, 1950·Science·A NOVICK, L SZILARD
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dong-Eun ChangTyrrell Conway
May 6, 2004·Journal of Bacteriology·Barbara MiddendorfJörg Hacker
Aug 11, 2004·The Journal of Biological Chemistry·Jean-Christophe BambouValérie Gaboriau-Routhiau
Sep 4, 2004·Applied and Environmental Microbiology·Patricia Escobar-PáramoRaymond Ruimy
Oct 22, 2005·International Journal of Medical Microbiology : IJMM·James R Johnson, Thomas A Russo
Oct 22, 2005·Infection and Immunity·M Chelsea LaneHarry L T Mobley
Aug 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elzbieta BrzuszkiewiczUlrich Dobrindt
Sep 6, 2006·Journal of Bacteriology·Andrew T Anfora, Rodney A Welch
Mar 30, 2007·BMC Biology·José-María Gómez-GómezJesús Blázquez
May 31, 2007·Proceedings. Biological Sciences·Karyn Meltz Steinberg, Bruce R Levin
Aug 19, 2007·Infection and Immunity·Shari A JonesTyrrell Conway
Nov 27, 2007·Molecular Microbiology·Fanette FontaineFrançois Taddei
Nov 28, 2007·Infection and Immunity·Jaroslaw ZdziarskiUlrich Dobrindt
Dec 21, 2007·PLoS Medicine·David A RosenScott J Hultgren

❮ Previous
Next ❯

Citations

Jul 29, 2011·The New England Journal of Medicine·David A Relman
Nov 20, 2012·Applied and Environmental Microbiology·Dominic FrigonRonald Gehr
Jun 3, 2011·Journal of Clinical Microbiology·Nuno MendonçaGabriela Jorge Da Silva
Dec 17, 2014·Journal of the Royal Society, Interface·R PopatS P Brown
May 29, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Petra SiškováVladana Woznicová
Dec 30, 2011·Current Opinion in Microbiology·Christopher J Alteri, Harry L T Mobley
Oct 11, 2011·International Journal of Medical Microbiology : IJMM·Christian-Daniel Köhler, Ulrich Dobrindt
Oct 7, 2011·International Journal of Medical Microbiology : IJMM·Bjørn-Arne Lindstedt
Nov 29, 2015·Molecular Biology and Evolution·David SkurnikErick Denamur
Jun 13, 2013·Environmental Microbiology Reports·Mathilde LescatErick Denamur
Mar 11, 2015·Environmental Microbiology Reports·Michaela D J BlytonDavid M Gordon
Mar 26, 2015·Molecular Biology and Evolution·Jerónimo Rodríguez-BeltránJesús Blázquez
Jan 24, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Roy R Chaudhuri, Ian R Henderson
Oct 22, 2011·Mobile Genetic Elements·Robert W JacksonJesús Murillo
Oct 24, 2014·Journal of Agricultural and Food Chemistry·Bingyong MaoWei Chen
May 24, 2015·Applied and Environmental Microbiology·Michaela D J BlytonDavid M Gordon
Mar 15, 2014·Nature Reviews. Microbiology·Richard C AllenSam P Brown
May 30, 2019·Antimicrobial Agents and Chemotherapy·Typhaine Billard-PomaresCatherine Branger
Jul 28, 2017·Microbes and Environments·Gitanjali NandaKafleVolker S Brözel
Sep 22, 2019·Applied and Environmental Microbiology·Sofiya G ShevchenkoEvgeni V Sokurenko
May 23, 2017·FEMS Microbiology Reviews·Médéric Diard, Wolf-Dietrich Hardt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.