Pathogenicity islands PAPI-1 and PAPI-2 contribute individually and synergistically to the virulence of Pseudomonas aeruginosa strain PA14.

Infection and Immunity
Ewan M HarrisonKumar Rajakumar

Abstract

Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and severe chronic lung infections in cystic fibrosis patients. The reference strains PA14 and PAO1 have been studied extensively, revealing that PA14 is more virulent than PAO1 in diverse infection models. Among other factors, this may be due to two pathogenicity islands, PAPI-1 and PAPI-2, both present in PA14 but not in PAO1. We compared the global contributions to virulence of PAPI-1 and PAPI-2, rather than that of individual island-borne genes, using murine models of acute pneumonia and bacteremia. Three isogenic island-minus mutants (PAPI-1-minus, PAPI-2-minus, and PAPI-1-minus, PAPI-2-minus mutants) were compared with the wild-type parent strain PA14 and with PAO1. Our results showed that both islands contributed significantly to the virulence of PA14 in acute pneumonia and bacteremia models. However, in contrast to the results for the bacteremia model, where each island was found to contribute individually, loss of the 108-kb PAPI-1 island alone was insufficient to measurably attenuate the mutant in the acute pneumonia model. Nevertheless, the double mutant was substantially more attenuated, and exhibited a lesser degree of virulence, than even PAO...Continue Reading

References

Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·H L RocchettaJ S Lam
Jan 20, 2000·Infection and Immunity·A KadiogluP W Andrew
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·M H Rashid, A Kornberg
Jan 5, 2002·Microbes and Infection·L A Knodler, B B Finlay
Nov 9, 2002·Biochimie·Yvon Michel-Briand, Christine Baysse
Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Matthew C WolfgangStephen Lory
Jan 2, 2004·Journal of Bacteriology·Jens KlockgetherBurkhard Tümmler
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jianxin HeLaurence G Rahme
Jul 28, 2004·Investigative Ophthalmology & Visual Science·Jing ZhangFu-Shin X Yu
Nov 24, 2004·Infection and Immunity·Ciara M Shaver, Alan R Hauser
Feb 8, 2005·American Journal of Respiratory and Critical Care Medicine·Ruxana T SadikotAlice S Prince
May 24, 2005·BMC Microbiology·Kyoung-Hee Choi, Herbert P Schweizer
May 16, 2006·Critical Care : the Official Journal of the Critical Care Forum·Evangelos J Giamarellos-BourboulisHelen Giamarellou
May 19, 2006·Journal of Bacteriology·Bridget R KulasekaraStephen Lory
Aug 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elzbieta BrzuszkiewiczUlrich Dobrindt
Sep 20, 2006·Infection and Immunity·Shelley N LuckElizabeth L Hartland
Oct 14, 2006·Genome Biology·Daniel G LeeFrederick M Ausubel
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Helen M MarriottDavid H Dockrell
Nov 24, 2006·Journal of Bacteriology·Mario JuhasDerek W Hood
Dec 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyun QiuStephen Lory
Dec 30, 2006·Journal of Bacteriology·Jens KlockgetherBurkhard Tümmler
Apr 7, 2007·International Journal of Medical Microbiology : IJMM·David W Wareham, Michael A Curtis
May 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lutz WiehlmannBurkhard Tümmler
Aug 24, 2007·FEMS Microbiology Letters·Dieco Würdemann, Burkhard Tümmler
Jan 19, 2008·American Journal of Respiratory and Critical Care Medicine·Timothy F MurphySanjay Sethi
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kalai MatheeStephen Lory

❮ Previous
Next ❯

Citations

Sep 16, 2011·The ISME Journal·Toshinari MaedaThomas K Wood
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Vanderlene L KungAlan R Hauser
Apr 13, 2011·PLoS Computational Biology·Matthew A OberhardtJason A Papin
Jan 8, 2016·Antonie van Leeuwenhoek·E Rodríguez-AndradeM I Ramírez-Díaz
Apr 16, 2016·International Journal of Antimicrobial Agents·Silvia Gómez-ZorrillaCarmen Peña
Feb 25, 2018·Microbial Ecology·Margarita StritzlerNicolás Ayub
Aug 30, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Albert GarretaXavi Carpena
Apr 5, 2017·The Journal of Infectious Diseases·Carlos JuanAntonio Oliver
Dec 24, 2018·Mobile DNA·João BotelhoLuísa Peixe
Sep 8, 2019·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·João BotelhoLuísa Peixe
Feb 15, 2021·Microbiological Research·Gertrudis Horna, Joaquim Ruiz
Apr 20, 2021·Frontiers in Cellular and Infection Microbiology·Erika Kushikawa SaekiGerson Nakazato
Jun 10, 2021·FEMS Microbiology Reviews·Rodrigo Ibarra-ChávezUrvish Trivedi
Jun 29, 2021·Virulence·Charles D MorinGee W Lau
Aug 20, 2021·Genes & Genomics·Luis F Espinosa-CamachoRosario Morales-Espinosa

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Related Papers

FEMS Microbiology Letters
Scott E BattleAlan R Hauser
Proceedings of the National Academy of Sciences of the United States of America
Lutz WiehlmannBurkhard Tümmler
© 2021 Meta ULC. All rights reserved