Pathogenicity of three type 2 porcine reproductive and respiratory syndrome virus strains in experimentally inoculated pregnant gilts

Virus Research
Andrea LadinigJohn C S Harding

Abstract

Mechanisms of reproductive failure resulting from infection with porcine reproductive and respiratory syndrome virus (PRRSV) are still poorly understood. Presented herein are the results of a side-by-side evaluation of the pathogenicity of three type 2 PRRSV strains in a reproductive model, from a pilot study used to develop experimental conditions and laboratory methods for a larger experiment. Pregnant gilts were experimentally infected with PRRSV at gestation day 85 or served as uninfected negative controls. After 21 days, all gilts and fetuses were necropsied. Clinical signs, litter outcome, viral load, cytokine levels, and pathology were compared from samples collected among pigs exposed to the three PRRSV strains. Based on differences in histologic lesions, and fetal weights, and numeric differences in gilt serum cytokine levels, litter outcome and virus replication in fetal tissues KS06-483 appeared less virulent than NVSL 97-7895 and KS06-72109 isolates. Levels of chemokine ligand 2 (CCL2), interferon alpha (IFNα), and interferon gamma (IFNγ) were increased in PPRRSV-infected compared to non-infected gilts (0.01 > P < 0.06). Inoculation with NVSL 97-7895 induced higher levels of all three cytokines. All three PRRSV isol...Continue Reading

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Citations

Dec 10, 2015·Annual Review of Animal Biosciences·Joan K LunneyGourapura J Renukaradhya
Dec 8, 2015·Nature Biotechnology·Kristin M WhitworthRandall S Prather
Apr 9, 2017·Molecular Reproduction and Development·Kristin M Whitworth, Randall S Prather
Apr 8, 2017·Veterinary Microbiology·Jack DekkersGraham Plastow
Mar 7, 2021·Vaccines·Chanhee Chae

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