Abstract
Murine congenital cytomegalovirus (CMV) (MCMV) infection of the inner ear cochlea, which caused continuous pathological change, occurred on the third day after intracerebral injection and persisted for a very long time. Our study used the MCMV-induced hearing loss neonatal mouse model to investigate the pathological changes of the cochlea in different time windows. Sixty newborn BALB/c mice were randomly and equally divided into the experimental group (MCMV intracerebral injection) and control group (normal saline intracerebral injection). At 1, 3, 5, 7, and 21 days after intracerebral injection, cochleas were extracted and evaluated by MCMV-DNA PCR analysis and histopathological examination. Two mice died before the end of the experiment in the experimental group, while there were no deaths in the control group. In the experimental group, the MCMV-DNA PCR samples did not show positive results in the gel electrophoresis until the 3-21 days after intracerebral injection, while no positive result was found in the control group. Pathological changes including hemorrhage and inflammatory cell infiltration of the scala vestibule and scala tympani began on the third day, spiral ganglion cell gap widening and cell thinning began on th...Continue Reading
References
Sep 1, 1990·American Journal of Otolaryngology·M Strauss
Oct 1, 1984·The Journal of Infectious Diseases·J P HarrisD D Richman
Aug 1, 1993·The Laryngoscope·K E Rarey, L E Davis
Apr 1, 1997·The Journal of Pediatrics·K B FowlerR F Pass
Aug 14, 2003·The Journal of Pediatrics·David W KimberlinUNKNOWN National Institute of Allergy and Infectious Diseases Collaborative Antiviral Study Group
Sep 10, 2003·Neurology·C ZuccaM Barbi
Feb 22, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jon D Reuter
Sep 6, 2005·Journal of Virological Methods·Yajarayma J Tang-FeldmanClaire Pomeroy
Dec 22, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Robert F PassSergio Stagno
Jun 21, 2006·Archives of Otolaryngology--head & Neck Surgery·David R WhiteMark R Schleiss
Jan 19, 2011·Archives of Otolaryngology--head & Neck Surgery·Stephanie MisonoBevan Yueh
Feb 16, 2011·Virology Journal·Chen JuanjuanChen Suhua
Mar 19, 2011·Journal of Neurovirology·Scott J SchachteleJames R Lokensgard
May 18, 2011·Cell Biochemistry and Biophysics·Yuehua QiaoHong Meng
Oct 18, 2011·Cell Biochemistry and Biophysics·Qiao YuehuaMeng Hong
Oct 29, 2011·Acta Neuropathologica·Natacha TeissierHoma Adle-Biassette